Abstract
Carrier ampholytes, peptides from protamine digested by α-chymotrypsin and two peptides, Gly–Gly–Gly and Gly–Gly–His, were labeled with dansyl chloride isoelectric point (pI) markers for evaluating pH formation in isoelectric focusing. These Dns-labeled ampholytes were partially purified by reversed-phase chromatography and/or preparative isoelectric focusing by rotatory cell and their pI values were estimated by comigration with protein-pI markers. These labeled markers were used to evaluate the resolution and the range of pH forming ability of carrier ampholytes.
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