Evaluation of ovarian reserve function in patients with systemic lupus erythematosus by anti-Mueller hormone

Abstract

Objective To evaluate the effect of anti-Mueller hormone on ovarian reserve in female patients with systemic lupus erythematosus(SLE). Methods The clinical data of 80 women with SLE in the Department of Rheumatism of Zaozhuang Municipal Hospital from June 2015 to June 2018 were collected.The patients used cyclophosphamide before entering the group were selected as A group(35 cases), and patients who didn't use cyclophosphamide were selected as B group(45 cases), and 30 healthy female volunteers were selected as the control group at the same time.All of them were normal menstrual cycle.At the third day of the menstrual cycle, the levels of anti-Mueller hormones were detected by enzyme linked immunosorbent assay.The serum levels of estrogen and alveolar hormone were detected by microparticle enzyme immunoassay, and the number of ovarian sinus follicles in each group was recorded by transvaginal ultrasound. Results The incidence rate of thrombocytopenia, nephritis, hemolytic anemia and neuropsychiatric manifestations in A group were 54.29%(19/35), 85.71%(30/35), 37.14%(13/35) and 22.86%(8/35), respectively, which were significantly higher than those in B group[48.89%(22/45), 53.33%(24/45), 13.33%(6/45), 0.00%(0/45)](χ2=6.33, 9.41, 6.16, 9.03, all P 0.05). The level of anti-Mueller hormone in patients with SLE aged>30 years[(0.98±0.31)g/L]was significantly lower than patients ≤30 years old[(1.86±0.60)g/L], and the level of anti-Mueller hormone in patients with cyclophosphamide accumulation>10 g[(0.36±0.11)g/L]was significantly lower than that of 10 g[(1.76±0.52)g/L](t=7.54, 11.30, both P<0.01). The results of Pearson correlation analysis showed that the anti-Mueller hormone showed negative correlation with the age of SLE and the accumulation of cyclophosphamide(r=-0.56, -0.45, both P<0.01). Conclusion The use of anti-Mueller hormone has good evaluation value for ovarian reserve function in female patients with SLE, and the level of anti-Mueller hormone is closely related to the age and the accumulation of cyclophosphamide.Therefore, cyclophosphamide should be used carefully in patients who are over 30 years old and the cumulative amount of cyclophosphamide over 10 g. Key words: Anti-mueller hormone; Lupus erythematosus, systemic; Ovarian function tests; Cyclophosphamide; Estrogens; Follicle stimulating hormone; Ovarian sinusoidal follicles; Age groups; Female