Evaluation of non‐clinical safety of Frutalin in adult Zebrafish (Danio rerio)

Abstract

Our previous studies have shown several biotechnological and biomedical activities of Frutalin (FTL), a lectin from Artocarpus incisa seeds. Its multiple specificity for α‐D‐galactose and a‐D‐mannose promotes affinity for glycans present in various cell receptors, such as ion channels. FTL presents isoforms, according to its deconvolved mass spectrum, which increases the biotechnological potential of the molecule, allowing the prospection of new applications. This study was conducted to evaluate the nonclinical safety of FTL in adult Zebrafish (Danio rerio). The action of FTL (1.0 mg/mL; 20 μL; ip) on the locomotor activity of adult zebrafish (Danio rerio) (ZFa; n = 6/group) was evaluated in the open field test.1 As controls were used one group without treatment (Naive) and another with vehicle (Saline 0,9%, 20 μL, ip). After 24 h of treatments, mortality was analyzed and the concentration able to kill 50% (LC50) of the zebrafish was calculated.2 The experimental procedures were approved by the Ethics Committee for the Use of Animals (CEUA), under protocol no. 7210149/2016, of the State University of Ceará (UECE), Fortaleza, Ceará. Despite interacting with other cellular receptors and promoting other pharmacological activities, FTL, active in the physiological environment of Zebrafish, did not modify the locomotor activity nor did it prove to be toxic to the animals, suggesting that its lectin activity, capable of promoting pharmacological activities when recognizing glycans of several ion channels, and membrane protein receptors, can not be associated with toxicity or locomotor damage.Support or Funding InformationThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.