Abstract
Two methods (I and II) for the evaluation of the phagocytic and bactericidal activities of neutrophil granulocytes in human disease are described by which the total number of viable bacteria and the number of viable intracellular bacteria in an in vitro phagocytic system are measured. In method I, the number of viable intracellular bacteria was determined using phenylbutazone for the inhibition of intracellular killing of bacteria and antibiotics for the inactivation of extracellular organisms. In method II, extracellular and intracellular bacteria were separated by differential centrifugation. The results obtained by method II were markedly obscured by contamination with extracellular bacteria. Method I provided a more accurate evaluation of the phagocytic and bactericidal activities of neutrophil granulocytes.
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