Evaluation of nano-scaled zero valent iron (nZVI) effects on continuous syngas biomethanation under the thermophilic condition

Abstract

The nano-scaled zero valent iron (nZVI) particles were applied to strengthen the syngas biomethanation under the thermophilic condition in a continuous bubble column reactor with gas circulation. The CH4 productivity was increased by 6.80% from 71.20 mmol∙Lr−1∙day−1 to the highest 76.04 mmol∙Lr−1∙day−1 at the nZVI concentration of 2.5 g/L. The measurement of iron concentration and the observation of the iron nanoparticles distribution indicate that nZVI can act as an electron conduit to enhance more efficient direct interspecies electron transfer by physically close contact between microorganisms, instead of biological corrosion. Further analysis of metabolic products shows that the nZVI addition can stimulate the EPS secretion, and the direct electron transfer relying on nZVI particles tends to replace other transfer modes. Microbial community analysis reveals that the Bacteria Bacteroidia and Firmicutes, and Archaea Methanothermobacter are the potential dominating enriched syntrophic partners. The expression of functional genes involved in methane production was also found to increase. On the other hand, the nZVI accumulation can lead to the albefaction and inactivation of partial sludge granules due to its toxicity. The negative effects of nZVI at high concentration are also more pronounced. This work shows the feasibility of improving continuous syngas biomethanation by strengthening interspecific association and accelerating electron transfer.