Abstract
In previous research, exhaled breath condensate (EBC) analysis has emerged as a promising noninvasive method for assessing human health, potentially increasing patient testing acceptance. However, current EBC collection devices require cooling equipment for temperature reduction, leading to issues such as increased size, increased energy consumption, and a lack of real-time collection capability. In this study, an EBC collector was developed that integrates controllable condensation microfluidics with a biomimetic water-collecting film. Microfluids induce an endothermic reaction by rapidly dissolving water and NH4NO3 within the chamber, reducing the surface temperature to 3.5 °C. When positioned inside a mask, exhaled air undergoes condensation and collection on the branched biomimetic film. Human tests were conducted to analyze caffeine and nicotine in the collected EBC samples using LC–MS, demonstrating the device’s advantages in terms of power-free operation, wearable design, and rapid sample collection for metabolite detection.
Published Version
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