Evaluation of Mn bioaccumulation and biosorption by bacteria isolated from spent nuclear fuel pools using54Mn as a radioindicator

Abstract

AbstractBioaccumulation and biosorption characteristics of Mn2+ions by both dead and living, non-growing biomass of Gram-positive bacteriaKocuria palustrisandMicrococcus luteusisolated from spent nuclear fuel pools were compared. The radioindicator method using radionuclide54Mn was applied to obtain precise and reliable data characterizing both processes as well as manganese distribution in bacterial cells. Manganese was mainly found on the surface (biosorption) of live cells of both bacteria and surface sorption capacity increased with Mn concentration in solution. Only 10.0% (M. luteus) and 6.3% (K. palustris) of uptaken Mn were localized in the cytoplasm (bioaccumulation). Biosorption of Mn by dead bacterial biomass was a rapid process strongly affected by solution pH. Maximum sorption capacitiesQmaxcalculated from the Langmuir isotherm and characterizing Mn binding represented 316±15 μmol/g forM. luteusand 282±16 μmol/g forK. palustris.Results indicate that living, non-growing cells showed a higher efficiency of Mn removal than dead biomass. Based on FTIR spectra examination with aim to characterize the surface ofK. palustrisandM. luteuscells, we confirmed that the phosphate and carboxyl functional groups are involved in manganese sorption onto cell surface by both live and dead bacterial biomass.