Abstract
One of the most recent studies in the field of genetics is to investigate the role of microRNAs as biomarkers for the diagnosis of multiple sclerosis. It is an inflammatory and degenerative disease of the central nervous myelin, which is manifested in numerous small and large plaques in the white matter of the brain and spinal cord. Formerly called has-miR-377-3p, miR-377-3p is located within the chromosomal region 32q14, and is located in the SOD gene. SOD (Superoxide Dismutase) is a gene located on chromosomal region 22q21 and the protein encoded by this gene is the superoxide dismutase enzyme. The aim of this study was to evaluate the expression of miR-377 in people with RRMS (Relapsing-Remitting Multiple Sclerosis) and healthy individuals in the Isfahan population. The study included 49 patients with RRMS and 52 healthy individuals who had no history of autoimmune and inflammatory disease. Total RNA was extracted from the blood lymphocytes of the study subjects using Ficol and Trizol and then made using miRNA cDNA, cDNA specific kit, and expression was measured by real-time RT PCR in healthy subjects and patients. Was. According to the results, miR-377-3p expression was higher in patients than in healthy subjects (P = 0.036) and the sensitivity and diagnostic value of miRNA was AUC = 0.80 (Area under the Curve). ) is. The results were consistent with previous studies and miR-377-3p could be used as a biomarker for the diagnosis of Multiple Sclerosis.
Highlights
Multiple sclerosis is an inflammatory disease of the central nervous system that is associated with the inflammation and disappearance of the nerve sheaths of the body called myelin; it is a sheath that surrounds nerve fibers and plays an important role in the rapid transmission of nerve waves
Superoxide dismutase (SOD) (Superoxide Dismutase) is a gene located on chromosomal region 22q21 and the protein encoded by this gene is the superoxide dismutase enzyme that is present in humans in three forms: SOD1, SOD2 and SOD3 [7]
SOD is a gene located on the chromosomal region 22q21 and the protein encoded by this gene is a superoxide dismutase enzyme that is present in humans in three forms: SOD1, SOD2 and SOD3 [7]
Summary
Multiple sclerosis is an inflammatory disease of the central nervous system that is associated with the inflammation and disappearance of the nerve sheaths of the body called myelin; it is a sheath that surrounds nerve fibers and plays an important role in the rapid transmission of nerve waves. Symptoms vary depending on demineralization sites and missing axons, including impaired vision, coordination, balance and movement [1]. The most common age for MS is between 20-40 years. Prevalence of MS in infancy is low after age 50[3]. MS is the second leading cause of youth disability in Iran, with an increase in the number of these patients per year (7, 7) [4]. The exact factors that cause MS are not known, the following factors may contribute to the development of MS, including DNA and mitochondrial dysfunction, viral infections, and hypoxia), Oxidative
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