Evaluation of luminol–H 2O 2–KIO 4 chemiluminescence system and its application to hydrogen peroxide, glucose and ascorbic acid assays

Abstract

A novel chemiluminescence (CL) system was evaluated for the determination of hydrogen peroxide, glucose and ascorbic acid based on hydrogen peroxide, which has a catalytic–cooxidative effect on the oxidation of luminol by KIO 4. Hydrogen peroxide can be directly determined by luminol–KIO 4–H 2O 2 CL system. The detection limit was 3.0×10 −8 mol l −1 and the calibration graph was linear over the range of 2.0×10 −7–6.0×10 −4 mol l −1. The relative standard deviation of H 2O 2 was 1.1% for 2.0×10 −6 mol l −1 ( N=11). Glucose was indirectly determined through measuring the H 2O 2 generated by the oxidation of glucose in the presence of glucose oxidase at pH 7.6. The present method provides a source for H 2O 2, which, in turn, coupled with the luminol–KIO 4–H 2O 2 CL reaction system. The CL was linearly correlated with glucose concentration of 0.6–110 μg ml −1. The relative standard deviation was 2.1% for 10 μg ml −1 ( N=11). Detection limit of glucose was 0.08 μg ml −1. Ascorbic acid was also indirectly determined by the suppression of luminol–KIO 4–H 2O 2 CL system. The calibration curve was linear over the range of 1.0×10 −7–1.0×10 −5 mol l −1 of ascorbic acid. The relative standard deviation was 1.0% for 8.0×10 −7 mol l −1 ( N=11). Detection limit of ascorbic acid was 6.0×10 −8 mol l −1. These proposed methods have been applied to determine glucose, ascorbic acid in tablets and injection.