Evaluation of Isochrysis galbana (clone T-ISO) cell numbers by digital image analysis of color intensity

Abstract

In microalgal cultivation, measuring cell numbers as a means to monitor growth rates is a long-standing problem. Many automated counting systems and schemes have been developed; among these are image analysis systems. However, such imaging systems have presented difficulties in dealing with the complexities of computer recognition of individual microscopic cells. It is known that the coloration of microalgae suspension is species specific and that color intensity increases are typically associated with increasing numbers. Using this qualitative insight, the present work describes the design, construction, and comparative performance of an inexpensive digital imaging system optimized for counting microalgal cells. The system circumvents the need to count individual cells and extracts cell numbers directly from the macroscopic color intensity of a microalgal suspension. The results suggest, using Isochrysis galbana (T-ISO) as an illustrative example, that this scheme is potentially useful for inexpensive and automated biomonitoring of microalgal cell numbers. Percentage difference comparisons with a standard Coulter Counter indicated that the three algorithms tested provided better than 10% accuracy over density thresholds of 1.52 × 106 to 8.10 × 106 cells mL−1 with precision of 4% attainable at high density concentrations.