EVALUATION OF ISCHEMIA MODIFIED ALBUMIN IN HEPATITIS C POSITIVE HEMODIALYSIS PATIENTS : A PILOT STUDY

V Pradeep Kumar,V Sivakumar,M.M Suchitra,P.V.L.N Srinivasa Rao,Aparna R Bitla,A Madhusudhana Rao

doi:10.7439/ijbar.v3i7.485

Abstract

Background: Chronic hemodialysis (HD) patients are more prone for infection such as Hepatitis C virus (HCV) infection, which may be associated with increased oxidative stress (OS). Ischemia-modified albumin (IMA) levels rises not only during ischemia but also during chronic OS. Hence the study was aimed to evaluate the levels of IMA and its association with oxidative stress in HCV positive HD patients. Methods: Twenty two HCV positive [HCV (+)], 22 HCV negative [HCV (-)] patients with end stage renal disease (ESRD) on maintenance HD and 22 healthy subjects were enrolled in this cross-sectional observational study. Plasma IMA was determined by spectrophotometric Co (II) - albumin binding assay. Plasma MDA was determined by spectrophotometric method. Results: Both MDA and IMA levels were significantly higher in HCV (+) and HCV (-) HD patients compared to controls (p 0.001). A significant increase in MDA levels was observed in HCV (+) HD patients when compared with HCV (-) HD patients (p 0.001); however no change in IMA levels were noted between HCV (+) and HCV (-) HD patients. A negative correlation was observed between IMA and MDA levels in both HCV (+) (r= - 0.278, p=0.178) and HCV (-) HD patients (r= - 0.193, p=0.354) which were however not statistically significant. Conclusion: Plasma IMA and MDA levels were elevated in both groups of HD patients when compared to controls. However, hepatitis C infection does not appear to cause any additional increase of IMA levels in HD patients

Highlights

Chronic hemodialysis (HD) patients are more prone for aquiring infection as HD requires vascular access for prolonged periods
Few studies have evaluated Ischemiamodified albumin (IMA) levels in end stage renal disease (ESRD) patients on HD and revealed a significant increase of IMA levels in HD patients compared to controls. 10, 11
AST and ALT were significantly increased in HD patients compared to controls, there was no significant diffrence between Hepatitis C virus (HCV) (+) and HCV (-) HD patients

Summary

Introduction

Chronic hemodialysis (HD) patients are more prone for aquiring infection as HD requires vascular access for prolonged periods. 8 The aggravated production of toxic oxygen radicals and related compounds has been noted in HD patients and patient with HCV infection and there is evidence of modification of albumin by both acute and chronic oxidative stress.[4, 9] Few studies have evaluated IMA levels in end stage renal disease (ESRD) patients on HD and revealed a significant increase of IMA levels in HD patients compared to controls. Chronic hemodialysis (HD) patients are more prone for infection such as Hepatitis C virus (HCV) infection, which may be associated with increased oxidative stress (OS). The study was aimed to evaluate the levels of IMA and its association with oxidative stress in HCV positive HD patients.

Methods

Results

Discussion

Conclusion