Evaluation of Inflammatory Serum Cytokines after Treatment with the Consciousness Energy Healing Based Proprietary Test Formulation on Combination of Cecal Slurry, LPS and E. Coli Induced Systemic Inflammatory Response Syndrome (SIRS) in Sprague Dawley Rats

The aim of this experiment was to evaluate the antioxidant and anti-inflammatory potential of the Biofield Energy Treated Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS and E. coli-induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats using lungs antioxidants and cytokines. In this experiment, various antioxidants like myeloperoxidase (MPO), superoxide dismutase (SOD), lipid peroxidation (LPO) and proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), macrophage inflammatory protein-2 (MIP-2) were analysed using ELISA assay in lungs. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of MPO was reduced by 45.77%, 53.36%, 47.80%, 53.89%, and 57.72% in the G5 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation); G6 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively as compared to the disease control (G2) group. Additionally, the level of SOD was increased by 7% in the G9 group as compared to the untreated test formulation (G4) group. The level of lipid peroxidation (LPO) end product in terms of malondialdehyde (MDA) was significantly reduced by 15.46% (p≤0.05), 14.53% (p≤0.05), and 11.88% in the G5, G6, G7, and G8 groups, respectively as compared to the G4 group. The level of TNF-α was decreased by 15.05%, 42.48% (p≤0.001), 48.73% (p≤0.001), and 42.85% (p≤0.001) in the G5, G6, G7, and G8 groups, respectively as compared to the G2 group. Moreover, the level of IL-6 was decreased by 29.44% (p≤0.001), 19%, 39.56% (p≤0.001), and 40.79% (p≤0.001) in the G5, G6, G7, and G8 groups, respectively as compared to the G2 group. Additionally, the level of MIP-2 was reduced by 10.99%, 14.37%, 40.16%, 16.75%, and 18.41% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G4 group. Overall, the data suggested the antioxidant and anti-inflammatory potentials of the Biofield Energy. Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Therefore, the results showed the significant slowdown the inflammation-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group.

The aim of this study was to evaluate the anti-inflammatory response of the Biofield Energy Treated Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS and E. coli-induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats using serum inflammatory biomarkers. In this experiment, various inflammatory biomarkers such as monokine induced by gamma interferon (MIG), regulated on activation, normal T cell expressed and secreted (RANTES), macrophage inflammatory protein-2 (MIP-2), matrix metallopeptidase 9 (MMP-9), troponin-1, procalcitonin, fibrinogen degradation product (FDP) were analysed using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The results showed that the level of MIG was significantly (p≤0.01) reduced by 48.57%, 56.14%, 47.95%, 51.92%, and 47.83% in the G5 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation); G6 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively as compared to the disease control (G2) group. Additionally, the level of regulated on activation, normal T cell expressed and secreted (RANTES) was significantly (p≤0.001) decreased by 47.35%, 46.32%, 40.73%, 50.47%, and 39.96% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. The level of macrophage inflammatory protein-2 (MIP-2) was significantly (p≤0.001) decreased by 91.74%, 93.54%, 92.70%, 94.46%, and 93.80% in the G5, G6, G7, G8, and G9 groups, as compared to the G2 group. The level of MMP-9 was significantly (p≤0.001) decreased by 18.74%, 35.54%, 26.48%, 38.16%, and 22.52% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Moreover, the level of troponin-1 was decreased by 28.53%, 28.50%, 14.14%, 30.68%, and 36.34% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the untreated test formulation (G4) group. Additionally, the level of procalcitonin was significantly (p≤0.001) decreased by 27.27%, 51.73%, 46.40%, 54.65%, and 39.08% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Further, the level of fibrinogen degradation product (FDP) was significantly (p≤0.001) decreased by 66.7%, 62.5%, 31.5%, 51.6% and 40.1% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested anti-inflammatory potentials of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Therefore, the results showed the significant slowdown the inflammation-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group.

The study was aimed to evaluate the antioxidant and anti-inflammatory activity of the Biofield Energy Treated Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS and E. coli-induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats. In this experiment, different antioxidants biomarkers such as myeloperoxidase (MPO), superoxide dismutase (SOD), lipid peroxidase (LPO) and proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), macrophage inflammatory protein-2 (MIP-2) were analysed using ELISA assay in intestines. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three groups of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of MPO was reduced by 21.16%, 15.9%, and 11.95% in the G5 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation); G7 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation from day -15); and G8 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15) groups, respectively as compared to the disease control (G2) group. Moreover, the level of SOD was increased by 30.34% (p≤0.001), 10.30%, and 13.62% in the G5, G6, and G8 group as compared to the untreated test formulation (G4) group. The level of lipid peroxidation (LPO) end product in terms of malondialdehyde (MDA) was significantly reduced by 23.58% (p≤0.001), 17.99% (p≤0.01), and 15.62% (p≤0.001) in the G5, G6, and G7 groups, respectively as compared to the G2 group. The level of TNF-α was decreased by 7.81% in the G5 group as compared to the G2 group. Moreover, the level of IL-6 was decreased by 38%, 40.4%, and 15.35% in the G7, G8, and G9 groups, respectively as compared to the G4 group. Additionally, the level of MIP-2 was reduced by 40.35%, 60.29%, 49.33%, and 42.43% in the G5, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested the antioxidant and anti-inflammatory potentials of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Therefore, the results showed the significant slowdown the inflammation-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group.

The aim of this experiment was to evaluate the antioxidants and anti-inflammatory potential of the Biofield Energy Treated/ Blessed Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS, and E. coli - induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats. The parameters like in brain (MMP- 9, FDP, Substance P, iNOS) and in heart (FDP, substance P, iNOS) were analysed using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of MMP-9 in brain was significantly ( p ≤0.001) reduced by 48.63%, 36.81%, 40.84%, and 60.03% in the G6 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli along with the Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively as compared to the untreated test formulation (G4) group. The level of FDP in brain was significantly reduced by 43.10% ( p ≤0.001), 33.40%, 35.46%, and 62.11% ( p ≤0.001) in the G6, G7, G8, and G9 groups, respectively as compared to the G4 group. The level of substance P was significantly decreased in brain by 18.42% ( p ≤0.001), 53.79% ( p ≤0.001), 16.65%, 14.64%, and 28.43% ( p ≤0.001) in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G4 group. The iNOS level in brain was significantly decreased by 35.92% ( p ≤0.001), 32.80%, 47.20% ( p ≤0.001), and 66.43% ( p ≤0.001) in the G6, G7, G8, and G9 groups, respectively than G4 group. Besides, FDP in heart tissue was significantly decreased by 15.66%, 17.41% ( p ≤0.05), 11.85%, and 13.02% in the G5, G6, G8, and G9 groups, respectively as compared to the G4 group. The levels of heart substance P and iNOS were altered. Overall, the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive maintenance groups showed an antioxidant and anti-inflammatory potentials. Therefore, the results showed the significant slowdown the inflammation- related disease progression and its complications/symptoms in the preventive groups ( viz. G6, G7, G8, and G9) that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries.

The aim of this experiment was to evaluate the anti-inflammatory potential of the Biofield Energy Treated Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS, and E. coli-induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats. The parameters studies in this experiment includes tumour necrosis factor alpha (TNF-α), interleukin-6 (IL-6), macrophage inflammatory protein-2 (MIP-2), matrix metallopeptidase 9 (MMP9), fibrin degradation products (FDP), Substance P, inducible nitric oxide synthase (iNOS) were analysed using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin E, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals received Biofield Energy Healing Treatment (prayer) remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of TNF-α was significantly (p≤0.001) decreased by 46.94% and 55.91% in the G6 (Cecal Slurry, LPS and E. coli along with Biofield Energy Treatment per se to animals from day -15) group as compared to the disease control group (G2) and the untreated test formulation (G4), respectively. The level of IL-6 was significantly (p≤0.001) reduced by 51.44% and 42.92% in the G6 group as compared to the disease control (G2) group and untreated test formulation group, respectively. The level of MIP-2 was significantly reduced by 21.52% and 31.54% (p≤0.001) in the G6 group as compared to the G2 and G4 groups, respectively. The level of MMP-9 was significantly decreased by 40.13% (p≤0.001) and 20.92% in the G6 and G8 groups, respectively as compared to the G2 group. Moreover, the level of FDP was significantly (p≤0.001) decreased by 38.22% and 50.53% in the G6 group as compared to the G2 and G4 groups, respectively. The level of substance P was significantly (p≤0.001) decreased by 36.96% and 38.20% in the G6 group as compared to the G2 and G4, groups, respectively. The level of iNOS was significantly (p≤0.001) decreased by 35.41% in the G6 group as compared to the G4 group. Overall, the data suggested the anti-inflammatory potentials of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Therefore, the results showed the significant slowdown the inflammation-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se group (G6) comparatively with the disease control group.

The aim of the present study was to evaluate the immunomodulatory action of Biofield Energy Healing (the Trivedi Effect®) Treatment to the animals and to the novel proprietary test formulation in tri-nitro benzene sulphonic acid (TNBS)-induced male Sprague Dawley rats. The test formulation consists of minerals (zinc, magnesium, iron, and copper) and vitamins (B6 , B12, and D3 ). Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Healing Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Additionally, three group of animals were also received Biofield Energy Treatment per se under similar conditions. The cellular biomarkers like cluster of differentiation-4 (CD4+ ), CD8+ , and CD28+ , biochemistry and hematology along with histopathologic profile were evaluated. The percentage of CD4+ count was increased by 8.87% in the G6 (Biofield Energy Treatment per se at day -15) group as compared to the untreated test formulation (G4) group. While the expression of CD8+ count was increased by 10.12% and 13.01% in the G6 and G7 (Biofield Energy Treated test formulation at day -15) groups, respectively as compared with the disease control (G2) group. Moreover, the level of CD28+ was significantly (p≤0.001) increased by 15.50% in the G5 (Biofield Energy Treated test formulation) group compared to the G2 group. In hematological analysis, platelet count was increased in the G7 by 8% compared with the G4. The level of uric acid was decreased by 40.47%, 13.8%, and 14.28% in the G6, G8 (Biofield Energy Treatment per se to animals plus Biofield Energy Treated test formulation at -15 day), and G9 (Biofield Energy Treatment per se to animals plus untreated test formulation) groups, respectively as compared with the G4 group. Histopathologic findings of all the tested groups did not show any abnormal findings with respect to the safe and nontoxic treatment strategies, which indicated that, the test formulation and Biofield Energy Treatment per se ameliorates colon inflammation in the TNBS-induced colitis rats by decreasing histopathologic lesions. Overall, the experimental data concluded that the Biofield Energy Treated test formulation showed considerable improved cellular and humoral immune response as compared with the untreated test formulation. Thus, the Trivedi Effect®-Biofield Energy Healing Treatment per se and the test formulation considerable improved cellular immune response, immunomodulatory effect, and gut health function.

The study was aimed to evaluate the antioxidant and anti-inflammatory activity of the Biofield Energy Treated Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on Cecal Slurry, LPS and E. coli-induced systemic inflammatory response syndrome (SIRS) model in Sprague Dawley rats. In this experiment, different antioxidants biomarkers such as myeloperoxidase (MPO), superoxide dismutase (SOD), lipid peroxidase (LPO) and cytokines like interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), macrophage inflammatory protein-2 (MIP-2) were analysed using ELISA assay in brain homogenate. A test formulation was formulated including minerals (magnesium, zinc, calcium, selenium, and iron), vitamin C, B6, E, B12, D3, β-carotene, cannabidiol isolate,and Panax ginseng extract. The component of the test formulation were divided into two parts; one section was defined as the untreated, while the other portion of each constituent and three group of animals received Biofield Energy Healing/Blessing Treatment remotely for about 3 minutes by Mr. Mahendra Kumar Trivedi, a renowned spiritual Energy Healer. The level of MPO was significantly (p≤0.001) reduced by 19.43%, 34.91%, 25.43%, 25.29% and 30.33% in the G5 (Cecal Slurry, LPS and E. coli + Biofield Energy Treated test formulation); G6 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli + Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se + Biofield Energy Treated/Blessed test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se animals + untreated test formulation) groups, respectively as compared to the untreated test formulation (G4) group. Moreover, the level of SOD was significantly increased by 45.02% (p≤0.001), 16.59%, and 35.99% (p≤0.001) in the G6, G7, and G9 groups, respectively as compared to G4 group. The level of TNF-α was significantly decreased by 12.66%, 46.92% (p≤0.001), 26.57% (p≤0.001), 23.22% (p≤0.001), and 54.28% (p≤0.001) in G5, G6, G7, G8, and G9 groups, correspondingly with reference to G4 group. Moreover, the level of IL-6 was significantly (p≤0.001) decreased by 37.51%, 20.28%, 21.55%, and 33.4% in the G6, G7, G8, and G9 groups, respectively as compared to the G4 group. Additionally, the level of MIP-2 was significantly (p≤0.001) reduced by 47.97%, 17.08%, 20.16% and 26.84% in the G6, G7, G8, and G9 groups, respectively as compared to the G4 group. Together, the data imply the antioxidant and anti-inflammatory potential of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Therefore, the results described a significant reduction of inflammation-related disease progression rate and its complications in the preventive maintenance groups (viz. G6, G7, G8, and G9).

The present experiment was evaluated to study of the effect of Consciousness Energy Healing Treatment (the Trivedi Effect ® ) on novel proprietary test formulation in male Sprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for the estimation of vitamin D3 metabolites in kidney tissue. A novel proprietary test formulation was formulated with a few minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The novel test formulation was divided into two parts; one part was defined as the untreated test formulation, while the other part of the test formulation and the animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. 25-OH vitamin D 3 level was significantly increased in the Biofield Energy Treated test formulation group (G5), Biofield Energy Treatment per se (G6), and 15-days pre-treatment of the Biofield Energy Treated test formulation (G7) groups by 21%, 22.9%, and 11.5%, respectively as compared with the disease control group (G2). Additionally, the level of 1,25-(OH) 2 D 3 was increased by 55% in the G6 group as compared with the G2. Further, the expression of CYP24A in kidney was increased by 13.9% in the G6 group, while CYP27B expression in kidney was significantly increased by 17.6%, 18.6%, 10.1%, and 15.6% in the G5, G6, G7 (Biofield Energy Treated test formulation from day -15), and G8 (Biofield Energy Treatment per se plus Biofield Energy Treated test formulation from day -15) groups, respectively as compared with the G4 group. Vitamin D receptor (VDR) expression was significantly increased by 69.5% and 31% in the G6 and G7 groups, respectively as compared with the G2 group. Overall, the results showed significant improvement of the availability of vitamin D 3 metabolites, that could be helpful for the vitamin D 3 deficiency persons to maintain good bone health and immunity. Therefore, the results suggest that there was significant slowdown in the disease progression and disease related all other complications/symptoms in the Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups ( viz . G6, G7, G8, and G9) comparatively with the disease group.

Vitamin D receptor (VDR) expression and telomerase enzyme activity was performed in the unpredictable chronic stress (UCS) model for the evaluation of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats using ELISA assay. A unique test formulation of minerals (Zn, Fe, Cu, Se, Ca, Mg), vitamins (C, E, B6 , B12, D3 ), and nutraceuticals (β-carotene, ginseng, and cannabidiol isolate) was formulated. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals were received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. VDR in liver showed an increased expression in the Biofield Energy Treated Test formulation to the untreated rats (G5), G7 (15-days pre-treatment of Biofield Energy Treated Test formulation), and G8 (15 days pre-treatment of Biofield Energy Treated test formulation to the Biofield Energy Treatment per se rats) groups by 16.7%, 75.7%, and 14.3%, respectively as compared with the untreated test formulation group (G4). Similarly, the VDR expression in kidney was increased by 22.3% and 13.6% in the G6 (Biofield Energy Treatment per se to the rats) and G8 groups, respectively, as compared with the G4. However, the VDR expression in heart was altered by 20.2%, 26%, and 28.2% in the G5, G7, and G8 groups respectively, as compared with the G4. The telomerase activity in the brain was significantly increased by 16.9%, 116%, 258.6% (p≤0.01), 148.1%, and 54.7% in the G5, G6, G7, G8, and G9 (untreated test formulation to the Biofield Energy Treatment per se to the rats) groups respectively, as compared with the G4. Similarly, telomerase activity in heart was also increased by 11.1% in the G8 group as compared with the G4. Overall, the experimental data suggested significance effect of Biofield Energy per se along with preventive measure on the animal with respect to various stress-related disorders. Overall, the results showed the significant slowdown the stress-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control and untreated test formulation groups

The present study aimed to evaluate the haematology, biochemistry, testosterone level, and histopathological analysis of Consciousness Energy Healing Treatment (the Trivedi Effect®) based test formulation in the unpredictable chronic stress (UCS) male Sprague Dawley (SD) rat model. The novel test formulation was composition of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. All the test formulation constituents were divided into two parts; one portion was defined as the untreated test formulation, while the other portion of the test formulation and the animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Haematology parameters such as red blood corpuscles (RBC) count was significantly (p≤0.001) improved by 6.61% and 9.37% in the Biofield Energy Treated Test formulation to the untreated rats (G5) and Biofield Energy Treatment per se to the rats (G6) groups, respectively as compared with the G2 group. The red blood cell distribution width (RDW-CV) level was significantly (p≤0.001) increased by 14.04%, 12.49%, and 11.85% in the 15 days pre-treatment of Biofield Energy Treated test formulation (G7), 15 days pre-treatment of Biofield Energy Treated test formulation to the Biofield Energy Treatment per se to rats (G8), and untreated test formulation to the Biofield Energy Treated rats (G9) groups, respectively as compared with the G2 group. The level of platelet count was significantly increased by 13.60% (p≤0.001) and 10.94% in the G6 and G9 groups, respectively as compared with the G2 group. Biochemical blood profile showed improved parameters with respect to magnesium, calcium, creatinine, phosphorus, uric acid, blood urea, sodium, potassium, and chloride level. Similarly, testosterone level was significantly increased by 476.39% (p≤0.05), 142.86%, 181.93%, and 234.46% (p≤0.05) in the G5, G6, G8, and G9 groups, respectively as compared with the untreated test formulation (G4) group. Overall, the data suggested that Biofield Energy Treatment per se showed significant improved blood profile along with preventive measure on the animal. Overall, the results showed the significant improve the human body immune responses, enhance resistance towards diseases, allergies, lethargic conditions, energy booster action, and its various immune deficiency diseases along with its associated complications/ symptoms can be preventive using Biofield Energy Treatment per se and/or Biofield Energy Treated Test formulation groups.

The experiment was aimed to evaluate the effect of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se on different vital functional serum biomarkers to the animals on L-NAME and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats. In this experiment, the functional serum biomarkers such as epinephrine/adrenaline, inducible nitric oxide synthase (iNOS), endothelial NOS (eNOS), plasminogen activator inhibitor-1 (PAI-1), intercellular adhesion molecule-1 (ICAM-1), glycated hemoglobin (HbA1c), and insulin were measured using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin B9 , vitamin B12, and vitamin D3 ), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one portion was referred as the untreated test formulation, while the other portion and three groups of animals received Biofield Energy Healing/Blessing Treatment remotely for about 3 minutes by a renowned spiritual healer, Mr. Mahendra Kumar Trivedi. The results showed that the level of adrenaline was reduced by 29.25%, 20.33%, 31.36%, and 41.97% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15), and G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15) groups, respectively as compared to the untreated test formulation group (G4). Moreover, the level of iNOS was reduced by 53.09%, 51.32%, 33.87%, 39.88%, and 40.02% in the G5, G6, G7, G8, and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively, as compared to the disease control group (G2). Additionally, the level of eNOS was increased by 83.65%, 94.93%, 71.48%, 48.64%, and 61.12% in the G5, G6, G7, G8, and G9 groups, respectively, as compared to the G2 group. The level of PAI-1 was decreased by 56.10%, 56.57%, 55.28%, 49.28%, and 57.84% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Besides, the level of ICAM-1 was decreased by 20.99%, 20.74%, 18.85%, and 17.89% in the G5, G6, G7, and G8 groups, respectively as compared to the G2 group. Level of HbA1c was decreased by 28.50%, 44.07%, 25.30%, 22.70%, and 48.79% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Further, the level of insulin was decreased by 26.09%, 21.24%, 5.41%, 23.61%, and 24.59% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested significance improvement of vital functional biomarkers of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various pathological conditions that might be beneficial various types of cardiovascular disorders. Therefore, the results showed the significant slowdown the inflammation-related cardiovascular disease progression and its symptoms in the preventive maintenance groups (viz. G6, G7, G8, and G9).

A novel proprietary formulation was designed that consist of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The study objective was to evaluate the impact of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for immunomodulatory activity. The test formulation was divided into two parts. One part was denoted as the untreated test formulation without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of total leukocytes count (TLC) were significantly increased by 23.1% and 17.44% in the Biofield Energy Treated test formulation (G5) and Biofield Energy Treated test formulation from day -15 (G7) groups, respectively compared with the disease control group (G2) induced by VDD. Moreover, the level of neutrophils was significantly increased by 71.68%, 92.92% (p≤0.01), 42.48%, and 49.56% in the G5, G7, Biofield Energy Treatment per se plus Biofield Energy Treated test formulation from day -15 (G8), and Biofield Energy Treatment per se animals plus untreated test formulation (G9) groups, respectively compared with the G2 group. Monocyte level was also increased by 23.53%, 17.65%, and 29.41% in the G5, Biofield Energy Treatment per se to animals from day -15 (G6), and G9 groups, respectively compared to the G2 group. Hepatic biomarker like alkaline phosphatase (ALP) was significantly reduced by 14.98% and 23.07% (p≤0.05) in the G7 and G9 groups, respectively as compared to the G2 group. Besides, cardiac biomarker like creatine kinase myocardium band (CK-MB) was also significantly (p≤0.05) reduced by 20.99% in the G6 group compared with the G2. Altogether, results suggested that the Biofield Treated test formulation and Biofield Energy Treatment per se significantly increased immune-related parameters, which might be beneficial for the management of immune-compromised patients as well as to boost-up the immunity in healthy peoples. The results showed a significant slowdown of disease progression and all other disease-related complications/symptoms in the preventive Biofield Energy Treatment group per se and the Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control group.

The aim of the study was to evaluate the immunomodulatory activity of the Biofield Treated/Blessed proprietary test formulation consisting of essential ingredients viz. minerals (zinc, magnesium, iron, and copper) and vitamins (B6, B12, and D3) in male Sprague Dawley rats. Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Healing Treatment/Blessing, while the other part was defined as the Biofield Energy Treated/Blessed sample, which received the Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi remotely. Additionally, three group of animals were also received Biofield Energy Treatment per se (at day -15) under similar conditions. The parameters were assessed such as immune biomarkers (IgM, IgG, IgA, IgE, CD4+, CD8+, and CD28+), biochemistry and hematology and histopathology. The experimental results showed IgG level was significantly increased by 10.70% and 8.03% in the G6 (Biofield Energy Treatment per se at day -15) and G8 (Biofield Treatment per se to animals plus Biofield Treated test formulation from day -15) groups, respectively as compared with untreated test formulation (G4). Additionally, CD8+ count was significantly increased by 20.67% in the G8 group, while CD28+ count was significantly increased by 11.70%, 8.32%, and 9.82% in the G7 (Biofield Energy Treated test formulation at day -15), G8, and G9 (Biofield Treatment per se (day -15) to animals plus untreated test formulation) groups, respectively after Biofield Energy Treatment to the animals as compared with the untreated test formulation. In hematological analysis, platelet count was increased in the G5, G6, G7, G8, and G9 groups by 40.69%, 27.95%, 26.67%, 38.58%, and 28.28%, respectively compared with the disease control (G2) group. Biochemical parameters showed significant decrease in the level of creatinine by 32.14% in the G9 group as compared with the G2 group. Further, animal body weight, feed intake, relative organ weight, and histopathological findings of all the tested groups did not show any abnormal findings with respect to the safe and non-toxic treatment strategies. Overall, the experimental data concluded that the Biofield Energy Treated/Blessed test formulation showed considerable improved cellular and humoral immune response as compared with the untreated test formulation. Thus, the Trivedi Effect®-Biofield Energy Healing Treatment per se and the test formulation has the significant capacity for immunomodulatory effect, stress management and anti-aging by improving overall health.

This present study evaluated the impact of Consciousness Energy Healing Treatment (the Trivedi Effect) on a novel test formulation in maleSprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for the estimation of thyroid biomarkers such as calcitonin, parathyroid (PTH),thyroid stimulating hormone (TSH), and thyroxine (T4). A novel proprietary test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The novel test formulation was divided into two parts; one part was defined as untreated test formulation, while the other of test formulation and the three group of animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of calcitonin (pg/mL) was significantly increased by 22.7%, 4.2%, 44.9%, 72%, and 67.7% (p≤0.01) in the Biofield Energy Treated test formulation group (G5), Biofield Energy Treatment per se (G6), 15 days pre-treatment of Biofield Energy Treated test formulation (G7), 15 days pre-treatment of Biofield Energy Treated test formulation to the Biofield Energy Treated animals (G8), and Untreated test formulation to the Biofield Energy Treated animals (G9) groups, respectively as compared with the untreated group (G4). However, the level of TSH was reported to be decreased by 23.3%, 20.4%, and 37.3% in the G7, G8, and G9 respectively as compared with the G4. The level of PTH was decreased by 30.6% and 10.9% in the G6 and G9, respectively, while it was increased by 9.7% in the G5 as compared with the G4. Similarly, the level of T4 was increased by 40.5%, 20.7%, and 20.8% in the G5, G7, and G8 groups respectively as compared with the G4. In conclusion, the Biofield Treated test formulation and Biofield Energy Treatment per se significantly improved the thyroid biomarkers in serum that can be used against many thyroid disorders such hyperthyroidism, goitre, thyroid nodules, thyroid cancer, thyroid hormone resistance, Hashimoto’s thyroiditis, and many more. Overall, the results showed the significant slowdown the disease progression and disease-related all other complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) compared with the disease control group. Keywords: Biofield Treatment; Thyroid Biomarkers; The Trivedi Effect; Thyroxine; Vitamin D3 deficiency diet; PTH; Thyroid Disorders

The study was aimed to investigate the potential benefits of the Consciousness Energy Healing Treatment (the Trivedi Effect®) per se and Biofield Energy Healing treated novel test formulation in male Sprague Dawley rats for their antiaging activity by monitoring aging biomarkers such as brain-derived neurotrophic factor (BDNF), silent information regulator-1 (SIRT-1), and klotho protein. The test formulation was distributed into two parts. First part did not provide any Biofield Energy Treatment was denoted as the untreated sample, however the second part was received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and defined as the Biofield Energy Treated sample. In this experiment, nine groups (n=10) were assigned, in which four were preventive maintenance groups. Among them, three groups of animals were also received Biofield Energy Healing Treatment per se (at day -15). BDNF was significantly increased by 25.83%, 19.35%, and 14.67% in the Biofield Energy Treated test formulation (G5), Biofield Energy Treatment per se at day -15 (G6), and Biofield Energy Treatment per se to animals plus Biofield Treated test formulation from day -15 (G8), respectively as compared to the disease control (G2) group. Moreover, expression of SIRT-1 protein was increased by 14.63% in the G5 group than the untreated test formulation (G4) group. Additionally, SIRT-1 activity was increased by 39.7%, 32.5%, 15.9%, and 136% in the G6, Biofield Energy Treated test formulation at day -15 (G7), G8, and Biofield Treatment per se (day -15) to animals plus untreated test formulation (G9) groups, respectively than the G4 group, while it was increased by 57.3% in the G9 group as compared to the G2 group. Besides, Klotho protein in kidney homogenate was significantly increased by 16.67% in the G5 group as compared to the G2 group. Altogether, the results showed a significant improvement of longevity mediators and antiaging biomarkers in the preventive maintenance groups. Therefore, results envisaged the significant slowdown of aging-related disorders and other complications in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group and could be utilized against various aging-related disorders like Alzheimer's disease, hypertension, osteoporosis, cataracts, type 2 diabetes, cancer, etc. along with it could be used to extend the life-span, stress and immune-related disorders.