Abstract
Passiflora mollissima L.H Bailey is an endemic species from the Andes mountain of Colombia in South America with important edible fruits and medicinal and economical properties. The development of improved micropropagation techniques is necessary to provide rapid and efficient clonal propagation of elite genotypes with high resistance and uniform production, as well as a system that can be used for genetic transformation. For this reason, the investigation focused on the evaluation of induction of somatic embryogenesis in P. mollissima from cotyledonary leaves, the effect of growth regulator concentrations and the orientation of explants on embryo production. Histological analyses of somatic embryogenesis were performed every 10 days after induction over 38 days of exposure to the medium. Results showed somatic embryo formation on Murashige and Skoog, (1962) culture medium supplemented with 4.5 μM 2,4-diclorophenoxyacetic acid plus 4.5 μM 6-benzyladenine. The results obtained are applicable to knowledge of non-zygotic embryogenesis in passionflower of the Andean region, for the purpose of improvement and commercial use. It is noteworthy that this is the first study in the induction and obtaining of embryos in P. mollissima.
Highlights
Passiflora is the largest and most important genus of the Passifloraceae family, comprising about 575 species [1]
Embryo formation is dependent on the species or genotype with which it is working [21]
It is important to highlight the key role of the use of cytokinins as BAP in 2.4D combination enhancing the formation of embryogenic calluses as indicated by [26] in cotyledons of Azadirachta indica
Summary
Passiflora is the largest and most important genus of the Passifloraceae family, comprising about 575 species [1]. In North America and Europe, the main species, P. incarnata, is popularly known as passion fruit or passion flower, while in South America, the most cultivated species are P. edulis var. P. mollissima is commonly known as ‘‘curuba de castilla’’ or ‘‘banana passion fruit”, and it grows as a vigorous climber in climates with average temperatures between 13 and 16°C. In Colombia, it is highly valued in the commercial market for its edible fruit and for its nutritional and medicinal properties. Production is destined to both local and international markets and the largest importer is Europe [4]
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