Abstract
28Feb 2017 EVALUATION OF IMMUNOGENICITY OF DNA VACCINE CODING OUTER MEMBRANE PROTEIN 31 (OMP31) OF BRUCELLA MELITENSIS IN MICE. Yasser M. Kamel , Noha A. Helmy , Amani A. Hafez and Safaa M. Barghash. Infectious Diseases Unit, Animal Health Department, Desert Research Center, Cairo, Egypt. Veterinary Serum and Vaccine Research Institute,Sera and Antigens Department, Abbasia, Cairo, Egypt. Parasitology Unit, Animal Health Department, Desert Research Center, Cairo, Egypt.
Highlights
Our work is aimed to evaluate vectored vaccine based on outer membrane protein 31 (OMP31) expressed by Escherichia coli (K12) as it is nonpathogenic strain and can deliver our protein to antigen presenting cells and promoting cellular immune response to control the infection with B. melitensisby using mice model [8]
Protection Experiments and evaluation of Brucella strain persistence:To evaluate the efficacy of vectored vaccine for protection against B.melitensis infection, five mice from each group was challenged by intra peritoneal route (I.P) with approximately 1x105 CFU/mouse of B.meletensis 16M
Humeral immunity response against B. melitensis:The serum immunoglobulin G (IgG) level was estimated in the mice of protection experiment up to day 28 post inoculation with either PBS, Rev1 attenuated vaccine or vectored vaccine
Summary
Our work is aimed to evaluate vectored vaccine based on outer membrane protein 31 (OMP31) expressed by Escherichia coli (K12) as it is nonpathogenic strain and can deliver our protein to antigen presenting cells and promoting cellular immune response to control the infection with B. melitensisby using mice model [8]. Protection Experiments and evaluation of Brucella strain persistence:To evaluate the efficacy of vectored vaccine for protection against B.melitensis infection, five mice from each group was challenged by intra peritoneal route (I.P) with approximately 1x105 CFU/mouse of B.meletensis 16M.
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