PRELIMINARY STUDY FOR PREPARATION OF COMBINED ATTENUATED VACCINE AGAINST SHEEP POX AND PPR VIRUSES

Zeidan S.M Zeidan,Namaa A Mohamed,El Dakhly A.T Dakhly,Olfat E Nakhla,Hanan M.S Elzawahr,Afaf A Abdel Wahab,Saad M.A.A Saad,Abeer A Tammam

doi:10.21474/ijar01/126

Abstract

30Apr 2016 PRELIMINARY STUDY FOR PREPARATION OF COMBINED ATTENUATED VACCINE AGAINST SHEEP POX AND PPR VIRUSES. Zeidan S.M , Namaa A. Mohamed , Hanan M.S.ELZawahry , Saad M.A.A , Olfat E. Nakhla , Afaf A.Abdel-wahab , Abeer A. Tammam and El-Dakhly A.T Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo,Egypt,Rinder pest like disease department. Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo,Egypt, Sheep pox department. Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt, Render pest department. Central Laboratories for Evaluation of Veterinary Biologics, Abbasia, Cairo,Egypt.

Highlights

The present study was performed to evaluatethe safety and immunogenicity of combined sheep pox (SP) and Peste des Petits ruminants (PPR) vaccine prepared in a lyophilized form
Evaluation of the prepared combined vaccine: The prepared vaccine was subjected to quality control testing including: Sterility tests: These tests include testing the freedom from bacterial; fungal and mycoplasma contaminants according to OIE (2014) Safety test: The safety of prepared combined vaccine was tested through inoculation of 5 sheep with a dose containing 105 TCID50 of each virus (100X field dose of the prepared vaccine) according to OIE (2012)
Virus titration: Both of sheep pox virus (SPV) and PPR viruses was titrated before mixing and lyophelization in Vero cells according to Rao and Malik (1982) and the virus titer was calculated according to Reed and Menuch (1938) as log 10 TCID50/ml

Summary

RESEARCH ARTICLE

PRELIMINARY STUDY FOR PREPARATION OF COMBINED ATTENUATED VACCINE AGAINST SHEEP POX AND PPR VIRUSES. Mohamed[2], HananM.S.ELZawahry[3], Saad M.A.A4, Olfat E. Nakhla[2], Afaf A.Abdelwahab[3], Abeer A. 1. Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo,Egypt,Rinder pest like disease department. 2. Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo,Egypt, Sheep pox department. 3. Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt, Render pest department. 4. Central Laboratories for Evaluation of Veterinary Biologics, Abbasia, Cairo,Egypt

Manuscript Info Abstract

DPV DPV DPV DPV

WPV WPV WPV WPV WPV WPV WPV