Abstract
The capacity of reversed-phase high-performance liquid chromatography (HPLC) and capillary gas chromatography (GC) to separate 37 sesquiterpene lactones of the Melampodiinae has been investigated. The HPLC system employed a Hypersil ODS column and methanol-water as eluent for isocratic and gradient separations. The GC system utilized a RSL-150 bonded phase capillary column with flame ionization detection. The previously characterized melampolide melnerin B was determined by both HPLC and GC to be a mixture of two isomers, containing a 2-methylbutanoate (melnerin B) or a 3-methylbutanoate (melnerin B′) substituent at C-8. The HPLC and GC methods developed have been applied to the analysis of a crude extract of Melampodium cinereum.
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