Abstract
A high performance liquid chromatographic (HPLC) method with UV detector for the determination of dyclonine hydrochloride and a gas chromatography (GC) method with flame ionization detector (FID) for the determination of camphor and menthol in lotion were developed. The developed HPLC method involved using a SinoChoom ODS-BP C18reversed-phase column (5 μm, 4.6 mm × 200 mm) and mobile phase consisting of acetonitrile : water : triethylamine in a ratio of 45 : 55 : 1.0; pH was adjusted to 3.5 with glacial acetic acid. The developed GC method for determination of camphor and menthol involved using an Agilent 19091J-413 capillary chromatographic column (30 m × 320 μm × 0.25 μm). The two methods were validated according to official compendia guidelines. The calibration of dyclonine hydrochloride for HPLC method was linear over the range of 20–200 μg/mL. The retention time was found at 6.0 min for dyclonine hydrochloride. The calibration of camphor and menthol of GC method was linear over the range of 10–2000 μg/mL. The retention time was found at 2.9 min for camphor and 3.05 min for menthol. The proposed HPLC and GC methods were proved to be suitable for the determination of dyclonine hydrochloride, camphor, and menthol in lotion.
Highlights
Dyclonine hydrochloride (Figure 1(a)), chemically 1-(4-butoxyphenyl)-3-1-piperidinyl)-1-ropanon hydrochloride is belonging to a bactericidal and fungicidal local anesthetic
Typical chromatograms of dyclonine hydrochloride, camphor, menthol, and blank excipients are shown in Figures 2 and 3. e analyte peaks were well resolved and free from tailing (
The HPLC method was linear over the range 20–200 μμg/mL for dyclonine hydrochloride. e regression equation YY Y YYYYYYY Y 157.56 was established based on the standard samples injected and their peak area with correlation coefficient of 0.9999
Summary
Dyclonine hydrochloride (Figure 1(a)), chemically 1-(4-butoxyphenyl)-3-1-piperidinyl)-1-ropanon hydrochloride is belonging to a bactericidal and fungicidal local anesthetic. E compound lotion is prepared with dyclonine hydrochloride, menthol, and camphor. Camphor, and menthol used as working standards were received as gi s from Zhengzhou Yonghe Pharmaceuticals Co., Ltd., Zhengzhou, China. E standard solution of dyclonine hydrochloride was prepared by dissolving accurately 10 mg pure drug in a 10 mL volumetric ask using mobile phase.
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