Abstract
Research on the parameters of full blood count and differential white blood count is included in the program of all medical laboratories of primary, secondary and tertiary health care levels. Today, all haematological tests are exclusively performed on the haematology analyzers. Automation of haematology laboratories is a result of the huge requires for haematological test performing, timely issuing of the haematological findings, and possibility of the usage of modern techniques. This work is an evaluation of laser haematology analyzer Cell-Dyn 3700 SL. It investigates the reliability of test results throughout the following parameters: precision, accuracy, sensitivity and specificity of determination methods. It also explores the influence of sample transferring and correlation with haematology analyzer MAXM Retti. Haematology parameters that have been investigated are: white blood cell (WBC), neutrophils (NEU), lymphocytes (LXM), monocytes (MONO), eosinophils (EOS), basophils (BASO), red blood cells (RBC), haemoglobin (HGB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCHC) red cell distribution width (RDW), platelet (PLT), mean platelet volume (MPV), plateletocrit (PCT), and platelet distribution width (PDW). The results confirms that precision of analyzer fulfils the reproducibility of testing parameters: WBC, RBC, HGB, MCV, MCH, MCHC, and PLT. Correlation coefficient values (r) gained throughout the statistical analysis, that is linear regression results obtained throughout the comparison of two analyzers are adequate except for MCHC (r = 0.64), what is in accordance with literature data. Accuracy is tested by haematology analyzer method and microscopic differentiating method. Correlation coefficient results for granulocytes, lymphocytes and monocytes point the accuracy of methods. Sensitivity and specificity parameters fulfil the analytical criteria. It is confirmed that haematology analyzer Cell-Dyn 3700 SL is reliable for the determination of full blood count in everyday work. Analyzer and its program for differential white blood count can be used for the research and separation of normal and pathological blood counts with addition of microscopic methods confirming distribution or morphologic changes of leukocytes.
Highlights
Laboratory investigation of the count, concentration and relative relation of haematological parameters is extremely important in clinical and other researches
There are 4 independent measurements: leukocyte counting and differentiation performed by optical principle, leukocyte counting performed by impedance principle in the current canal, erythrocyte and platelet counting performed in the independent canal and haemoglobin measurement performed by spectrophotometer
Measurement of the the same sample (N=25) according to haematological parameters: white blood cell (WBC), red blood cells (RBC), HGB, HCT, mean corpuscular volume (MCV), MCH, MCHC and PLT has been performed for the precision testing
Summary
Laboratory investigation of the count, concentration and relative relation of haematological parameters is extremely important in clinical and other researches. Manual techniques have been performed in haematological laboratories for a long time. These techniques are slow and strenuous while their subjectively based test estimation and work precision like white blood cells count on haemocytometer are not on the acceptable level. Microprocessors and computers, as integral part of haematological counter, induced a sudden development of automation and an expansion of different types of haematological tests (2). New generation of haematology analyzers enable fast and reliable obtaining of the full blood count data and differential white blood count information screening. Data on abnormalities in distribution, that is changes in relations between normal leukocyte types helps in diagnosing and following up of different disorders such as haematological and infectious disease processes
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