Abstract
We have evaluated the ‘Mixture Analysis’ tool in GeneMapper ® ID-X v1.1 (Applied Biosystems) to interpret two-person DNA mixtures. This package depends upon the evaluation of two different parameters – the mixture proportion ( M x) and the heterozygote balance ( H b). We analysed 3000 heterozygotes in order to determine the characteristics of heterozygote balance versus the average peak height ratio (PHR). This information was used to determine thresholds that were subsequently used to accept or to reject mixture genotypes. Apart from the evaluation of likelihood ratios conditioned on suspect's and victim's reference samples, the module can also be used to deconvolve mixtures. This may be important if a suspect or reference sample is not available for analysis. A case example was analysed in order to illustrate the principles. This evaluation forms the basis of a validation exercise to use the GeneMapper ® ID-X Mixture Analysis tool in forensic casework at the Department of Criminal Forensic Genetics.
Published Version
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