Abstract
Gas explosion can lead to serious global public health issues. Early period gas explosion injury (GEI) can induce a series of histopathologic and specific metabolic changes. Unfortunately, it is difficult to treat GEI thoroughly. To date, the specific molecular mechanism of GEI is still unclear. To accurately diagnose and provide comprehensive clinical intervention, we performed a global analysis of metabolic alterations involved in GEI. The physiological and behavioral indicators' changes of rats after gas explosion were observed. These metabolic alterations were first investigated in a rat model using serum metabonomics techniques and multivariate statistical analysis. Significant heart rate (HR), mean blood pressure (mBP), and neurobehavioral index changes were observed in the GEI group after gas explosion. UPLC-MS revealed evident separated clustering between the control and GEI groups using supervised partial least squares discriminant analysis (PLS-DA). We designed an integrated metabonomics study for identifying reliable biomarkers of GEI using a time-course analysis of discriminating metabolites in this experiment. The metabonomics analysis showed alterations in a number of biomarkers (21 from serum). The meaningful biomarkers of GEI provide new insights into the pathophysiological changes and molecular mechanisms of GEI, including the disturbances in oxidative stress and neuroinflammatory reaction, as well as in metabolism of lipids, glucose, and amino acids in rats, suggesting that the process of GEI in humans is likely to be comprehensive and dynamic. Correlations between the GEI group and the biomarkers identified from the rat model will be further explored to elucidate the metabolic pathways responsible for GEI in the human body.
Highlights
In recent years, the number of major disasters, such as gas explosions, has been increasing worldwide [1]
We observed decreased motion distance and trajectory of rats, while residence time was increased in the gas explosion injury (GEI) group in an open-field experiment, indicating that gas explosion could result in brain nerve and impaired the neurological behavioral function in rats (Figure 2)
Serum metabonomics based on Ultraperformance liquid chromatography (UPLC)-mass spectrometry (MS)/MS has been of great value in the discovery of biomarkers and the elucidation of the pathogenesis of various diseases [19]; we hypothesized that a similar method could be used to evaluate GEI
Summary
The number of major disasters, such as gas explosions, has been increasing worldwide [1]. Gas explosion accidents result in substantial direct and indirect economic loss in China every year, which could severely limit the development of the coal industry [2]. Gas explosion is a common accident in the coal mine industry that seriously threatens the state of the property and the safety of miners. BioMed Research International to be greatly limited, resulting in an occasional happening of vicious gas explosion [3]. Gas explosion can generate shock wave and heat that cause injury and aggravates wounds via the interaction of blast and burns [4]. In the coal mine roadway, workers are directly exposed to the explosion environment due to the lack of protection for the body, which greatly increases the probability of injury caused by the blast wave. Blast wave is very likely to lead to severe damage to the whole body
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
