EVALUATION OF FREE RADICAL SCREENING AND ANTIOXIDANT POTENTIAL OF MORINGA CONCANENSIS NIMMO-A MEDICINAL PLANT USED IN INDIAN TRADITIONAL MEDICATION SYSTEM

Brindha Banu Balakrishnan,Kalaivani Krishnasamy

doi:10.22159/ijpps.2018v10i7.26403

Brindha Banu Balakrishnan, Kalaivani Krishnasamy

Open Access

https://doi.org/10.22159/ijpps.2018v10i7.26403

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Abstract

Objective: We aimed to investigate the free radical scavenging, antioxidant and hepatoprotective potential of M. concanensis Nimmo leaves.Methods: Free radical scavenging activity was evaluated by employing various accepted in vitro systems, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), hydroxyl (OH) and nitric oxide (NO) radical. Antioxidant potential of M. concanensis Nimmo extract was assessed against H2O2 in goat liver by determination of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glucose-6-phosphatase dehydrogenase (G-6-PDH), total reduced glutathione (GSH), vitamin C, vitamin E activity, and lipid peroxidation (LPO).Results: Results showed that the amount of plant extract of M. concanensis Nimmo required to scavenge 50% of the DPPH radicals was 401.80 μg/ml, ABTS radical was 353.14 μg/ml, OH radical was 433.71 μg/ml and NO radical was 371.24 μg/ml. Also, the pre-treatment of ethanolic extract of M. concanensis Nimmo leaves in goat liver showed a significant protection against H2O2 induced oxidative stress by retaining of antioxidants of SOD (4.76±0.25), CAT (26.81±0.25), GPx (6.41±0.34), GST (2.31±0.10), G-6-PDH (0.84±0.02), GSH (37.30±1.12), vitamin C (3.01±0.07), vitamin E (14.43±0.45) within normal range.Conclusion: In conclusion, promising free radical scavenging, the antioxidant activity of M. concanensis Nimmo leaves can be able to treat various diseases caused by free radicals.

Highlights

The liver plays an essential role in the metabolism of xenobiotics, catabolism, biochemical process, physicochemical functions of the body like oxidation, reduction, hydroxylation, hydrolysis, etc
Free radicals are spontaneously produced as byproducts under certain environmental conditions in the biological systems during the normal metabolic process which may cause extensive damage to tissues and biomolecules by lipid peroxidation, breakdown of deoxyribonucleic acid (DNA) strands, denaturation of proteins and disrupting cellular functions leading to various diseases including cancer, diabetes mellitus, chronic inflammation, and neurodegenerative disorders [31]
In this study, we determined the antioxidant property of M. concanensis Nimmo leaves against DPPH, ABTS, OH and nitric oxide (NO)-free radicals

Summary

Introduction

The liver plays an essential role in the metabolism of xenobiotics, catabolism, biochemical process, physicochemical functions of the body like oxidation, reduction, hydroxylation, hydrolysis, etc. Some hepatotoxic agents cause the liver damage is of severe consequences. Oxidative stress is causative agents for various ailments include deoxyribonucleic acid (DNA) damage, cancer, and degeneration of cellular membrane leading to diabetes, liver diseases [2]. The production of oxidative stress can be monitored by an antioxidants system which is robust agents scavenge the free radicals and promotes their decomposition and suppresses their mechanism [3]. The model was very carefully designed to simulate in vivo intraperitonial exposure of oxidant. This study was prepared based on the recommendation by the fund for the replacement of animals in medical experimentation (FRAME) to diminish the use of live animals in research and to develop a model system that would simulate in vivo conditions [4]

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