Abstract
Histopathological analysis is one of the primary pillars in clinical diagnostics. The efforts to implement optical techniques aim at alleviating the burden of delivering timely and accurate diagnoses. We have explored the potential application of unstained tissue slides’ autofluorescence to differentiate collagen-related skin degenerative diseases, such as psoriasis, lupus erythematosus, scleroderma, and Syndrome of Raynaud. This exploration involved two techniques: fluorescence microscopy combined with colorimetric analysis and synchronous fluorescence spectroscopy. We addressed the main characteristic peculiarities of the examined samples and discussed the evaluation of potential classification parameters along with their diagnostic values.
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