Abstract
BackgroundHematopoietic evaluation of the patients after Hematopoietic stem cell transplantation (HSCT) is very important. Erythroblast macrophage protein (Emp) is a key protein with function in normal differentiation of erythroid cells and macrophages. Emp expression correlates with erythroblastic island formation, a process widely believed to be associated with hematopoiesis in bone marrow. We aimed to investigate the hematopoietic function of bone marrow from 46 HSCT patients and 16 inpatients with severe anemia applied to the treatment of EPO by measuring Emp expression level.MethodsEmp mRNA and protein expression levels in mononuclear cells of bone marrow and peripheral blood samples were detected by RT-PCR and Western blotting method respectively.ResultsWhile hematopoiesis occurs in bone marrow, Emp expression level was elevated and more erythroblastic islands were found , and Emp is upregulated in bone marrow in response to erythropoietin (EPO) treatment.Conclusions Emp expression correlates with erythroblastic island formation and has an important function for bone marrow hematopoiesis. Emp could be a potential biomarker for hematopoietic evaluation of HSCT patients.
Highlights
Hematopoietic evaluation of the patients after Hematopoietic stem cell transplantation (HSCT) is very important
We suggest that Erythroblast macrophage protein (Emp) is a potential biomarker for hematopoietic evaluation of HSCT patients
The morphological characteristics of bone marrow were described by the bone marrow hyperplastic degree (BMHD) and number of erythroblastic islands
Summary
Hematopoietic evaluation of the patients after Hematopoietic stem cell transplantation (HSCT) is very important. The erythroblastic island is a distinct anatomic unit which consists of a central macrophage surrounded by erythroid cells. These islands are present at various stages of differentiation in the fetal liver and the adult bone marrow, and have a key role in erythroid cell proliferation and differentiation [1,2,3,4]. Found that loss of Emp function in erythroid cells resulted in impaired proliferation and terminal differentiation[18]. These findings suggest that Emp protein might be a potential molecular marker for hematopoietic evaluation
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