Evaluation of Efficacy and Potential Complications of the Plant-Based Nanoparticles Hemostat Powder on Controlling Hemorrhage in Rat Models of Liver Injury: An Experimental Study

Abstract

BackgroundLiver surgery is associated with an increased risk of bleeding, which required time management. Several hemostatic agents have been developed. However, plant-based materials have shown some superiority in hemostasis time and lower complications. The aim of our study was to prepare plant-based topical hemostatic Nanoparticles from the rosaceous family as a novel hemostatic agent.Material and Methods Twenty male Wistar rats were randomly divided into two study groups. All the rats were sedated and underwent incision in the anterior lobe of the liver. In the control group, the incision site was firstly compressed with a manual compression with dry gauze for 2 minutes and then repeated every 30 seconds until hemostasis was achieved. In the intervention group, homeostatic material was applied on the site of the incision, and clotting time was recorded. Paired Samples Test was used to compare the mean hemo­stasis times in Nanoparticles and control groups. P <0.05 considered significant.ResultsHemostasis was successfully achieved in both groups. Hemostasis time in Nanoparticles and control groups were 6.7±1.33 and 183±26.26 seconds, respectively and their differences were significant (P < 0.001). The histopathology assessment showed mild increases in vascularity, fibrosis, and polymorph nuclear neutrophils (PMN) infiltration was seen in one case. Mild to moderate lymphocyte infiltration was detected in 2 cases. ConclusionThe produced plant-based Nanoparticles could significantly reduce the hemostasis time compared to the control group.