Evaluation of effect of local exogenous application of Myrrh oil on healing of wound incisions of facial skin (Histochemical, Histological and Histomorphometrical study in rabbits)

Nada M Al-Ghaban,Nawar Bahjet Kamil

doi:10.26477/jbcd.v31i4.2724

Abstract

Aim of the study: Is to evaluate the effect of myrrh oil local application on the healing process of skin wounds histologically , histomorphometrically and , histochemically. Materials and methods:Twenty male white New Zealand rabbits were used in this study. An incisional wounds with full thickness depth and of 2 cm length were done on both sides of the cheek skin of each rabbit. The left sided incisions (the control group) were irrigated with distilled water (10µL). The right sided incisions (the experimental groups) were treated with myrrh oil (10µL). Each group was subdivided into 4 subgroups according to the healing interval into 1,3,7 and 14 days(5 rabbits for each group). Results: Histological findings of our current study showed a highly significant difference between the experimental and the control groups in count of the inflammatory cells which showed that mean values increased with time for the control and the experimental groups. The histomorphometrical findings had shown that the thickness of the epithelium was nearly completed at about 7 days for the experimental groups and at about 14 days for the control. The blood vessels count was recorded to have a highly significant difference between the groups at days 1 and 3 only. The histochemical findings had shown that the collagen fibers remodeling had recorded a highly significant difference between the control and the experimental groups at days 7 and 14. Conclusion: The current study had revealed that myrrh oil accelerates wounds healing in rabbits. Key word:Wound healing , Myrrh oil, Masson’s Trichrome staining.

Highlights

The skin can be defined as an interface or a barrier between the body and the external environment [1]
Inflammatory cell parameter The results showed as in table [2] that the highest mean values were recorded for the experimental groups at day 3 and the lowest mean value was at 14 days
The epithelial thickness parameter: results had revealed as shown in table [3] that the epithelial thickness highest mean value for the control and the experimental groups was recorded at day 14 and that the lowest mean value for both both groups wasrecorded at day 1, it showed a highly significant differences between the control and the experimental groups at all healing period intervals (p

Summary

INTRODUCTION

The skin can be defined as an interface or a barrier between the body and the external environment [1]. The process of wound healing, which is considered a normal biological process of the body, can be achieved by four highly programmed, strict and precise phases. The animals were randomly divided in accordance to the healing intervals into 4 groups [1,3,7,14] (5 rabbits in each group) and each group was subdivided in to: 1- Control group: The left sided incisions were irrigated with distilled water. 4. Collagen fiber density analysis: The slides were stained with masson’s trichrome stain and were examined under the light microscope, power x40, at 3,7 and 14 days period intervals with the help of image J software [13]

RESULTS

Findings

DISCUSSION

CONCLUSION