Abstract
Pigs were delivered by cesarean at 106 or 114d of gestation and fitted with umbilical catheters. Pigs received either a control, a low DHA/AA (0.3 and 0.6% of total lipids as DHA and AA, respectively), or a high DHA/AA (5% and 11 % of total lipids as DHA and AA, respectively) total parenteral diet for 6d. Hepatic CPT activity was measured before and after malonyl‐CoA inhibition. There was a tendency for increased total CPT activity in preterm compared to term pigs receiving the control diet (p = 0.07) and was attributed to malonyl‐CoA insensitive activity, which was elevated across dietary treatments in preterm versus term pigs (p<0.05). This increased CPT activity was concomitant with increased CPT I transcript amounts (p < 0.05). Whereas the low DHA/AA diet resulted in postnatal upregulation of hepatic CPT I mRNA in term pigs, the control and high DHA/AA diet elicited a similar response in CPT I mRNA in preterm pigs (p < 0.05). It is unlikely that the differences observed in CPT can be ascribed to PPARα as transcript amounts were increased in term pigs receiving the control and low DHA/AA diets (p < 0.05), yet postnatal changes in PPARα were not observed in preterm pigs receiving the high DHA/AA diet. Collectively, these findings suggest that postnatal induction of hepatic CPT I is more robust in preterm pigs. This research has been supported by funds appropriated by the Ohio General Assembly to The Ohio State University, Ohio Agricultural Research and Development Center.
Published Version
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