Evaluation of different operational conditions in a microbial electrolysis cell inoculated with a pure culture of Shewanella oneidensis for hydrogen production.

Abstract

Hydrogen is a promising alternative to meet the world's energy demand in the future because of its energetic characteristics. Microbial electrolysis cell (MEC) produces hydrogen from organic matter using exoelectrogenic bacteria. Shewanella oneidensis stands out for having the capacity to produce hydrogen using different electron transfer mechanisms. The present research aims to evaluate the hydrogen production efficiency in a MEC inoculated with a pure culture of S. oneidensis in different operational conditions. Since the use of a catalyst accounts for most of the MEC cost, no catalyst was used for anode or cathode. Experiments were performed in semi-continuous and batch mode using different electrodes, voltages applied, and medium in aerobic and anaerobic conditions. The highest hydrogen production rate (HPR) was 0.107 m3 of H2/m3day obtained in a semi-continuous experiment using graphite plates and stainless steel electrodes. In batch experiments, a HPR occurred at 0.7V, with a value of 0.048 m3 of H2/m3day versus 0.037 m3 of H2/m3day with 0.9V. HPR was higher with carbon felt electrode (0.056 m3 of H2/m3day). However, current density dropped after 38h, with carbon felt electrodes, and did not recover. Results of the present research showed that the MEC using a pure culture of S. oneidensis can be considered an alternative for hydrogen production without using a catalyst. Also, S. oneidensis produced hydrogen in both anaerobic and aerobic conditions with low methane production. Optimization can be proposed to improve hydrogen production based on the operational conditions tested in these experiments.