Abstract
Background Mammary gland neoplasms are the most prevalent tumors in dogs, and their treatment is still challenging. A crucial problem in the handling of this type of neoplasm is to obtain primary mammary epithelial cell cultures from the original tumors. The aim of this study was to determine the best conditions to culture primary mammary epithelial cells from several histological types of canine breast tumors.
Highlights
Mammary gland neoplasms are the most prevalent tumors in dogs, and their treatment is still challenging
simple adenoma. The cells were phenotypically characterized according to an immunocytochemical panel
Results have shown that the best method of obtaining primary epithelial cell lines comprises the use
Summary
Evaluation of different methods to obtain primary mammary epithelial cell cultures from canine spontaneous mammary gland tumors. Luciana B Gentile[1], Márcia K Nagamine[1], Isis P Jesus[1], Fábio T Toyota[2], Adriana T Nishiya[3], Tatiane M Giovani[4], Daniel S Sanches[1], Heidge Fukumasu[4], Maria Lúcia Z Dagli1*. From São Paulo Advanced School of Comparative Oncology Águas de São Pedro, Brazil. From São Paulo Advanced School of Comparative Oncology Águas de São Pedro, Brazil. 30 September - 6 October 2012
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