Abstract
Objective: Microorganisms, especially bacteria and its proteins have proven to be potential anti-cancer agents as they selectively attack the tumor cells or tumor micro-environments. The extract of Pseudomonas aeruginosa found to contain proteins that have shown promising anticancer activity. In this work, it was attempted to increase the biomass and trigger the total protein fraction of Pseudomonas aeruginosa (MTCC 2453).Methods: The organism was cultivated in three different such as Luria-Bertani (LB) broth, minimal medium9 (M9), super broth medium (SB) and asparagine-proline (AP) broth. Asparagine proline broth was selected as it has shown high cell growth rate. The media was further optimized by the addition of NaHCO3 and copper sulphate to trigger the protein production. Optimized Aspergine proline broth has achieved highest cell biomass. After the shake flask culture, the overnight grown culture in optimized AP medium was further grown in a 5 L bioreactor by fed-batch cultivation to achieve higher cell densities.Results: The highest protein production was achieved at 40 ° C. Highest biomass and protein content was observed at pH 8 while lowest biomass was produced at pH 2. A gradual increase in biomass content observed from 12 h towards to 48 h.Conclusion: High biomass and proteins content and of Pseudomonas aeruginosa (MTCC 2453) can be produced in optimized asparagine-proline broth. Further the extract is purified to produce novel anti-cancer proteins.
Highlights
Today, cancer treatment is facing difficulties with secondary effects caused by standard therapies and affected by tumor cell resistance
Bacterial proteins have been extensively studied as anticancer agents [2]
The media was further optimized by the addition of NaHCO3 and copper sulfate to trigger the protein production
Summary
Cancer treatment is facing difficulties with secondary effects caused by standard therapies and affected by tumor cell resistance. Bacterial proteins are studied as one of the potential strategies to treat cancer. Live, attenuated bacteria have shown the ability to act as anticancer agents [1]. Sometimes they may cause infections and cross contamination reactions. Bacterial proteins have been extensively studied as anticancer agents [2]. These proteins found to act on tumor cells and cause tumor regression through growth inhibition; cell cycle arrest and apoptosis induction [3]. Bacillus thuringiensis found to contain a new anticancer protein [5]
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