Abstract
BackgroundRecent studies have demonstrated that microalga has been widely regarded as one of the most promising raw materials of biofuels. However, lack of an economical, efficient and convenient method to harvest microalgae is a bottleneck to boost their full-scale application. Many methods of harvesting microalgae, including mechanical, electrical, biological and chemical based, have been studied to overcome this hurdle.ResultsA new flocculation method induced by decreasing pH value of growth medium was developed for harvesting freshwater microalgae. The flocculation efficiencies were as high as 90% for Chlorococcum nivale, Chlorococcum ellipsoideum and Scenedesmus sp. with high biomass concentrations (>1g/L). The optimum flocculation efficiency was achieved at pH 4.0. The flocculation mechanism could be that the carboxylate ions of organic matters adhering on microalgal cells accepted protons when pH decreases and the negative charges were neutralized, resulting in disruption of the dispersing stability of cells and subsequent flocculation of cells. A linear correlation between biomass concentration and acid dosage was observed. Furthermore, viability of flocculated cells was determined by Evans Blue assay and few cells were found to be damaged with pH decrease. After neutralizing pH and adding nutrients to the flocculated medium, microalgae were proved to maintain a similar growth yield in the flocculated medium comparing with that in the fresh medium. The recycling of medium could contribute to the economical production from algae to biodiesel.ConclusionsThe study provided an economical, efficient and convenient method to harvest fresh microalgae. Advantages include capability of treating high cell biomass concentrations (>1g/L), excellent flocculation efficiencies (≥ 90%), operational simplicity, low cost and recycling of medium. It has shown the potential to overcome the hurdle of harvesting microalgae to promote full-scale application to biofuels from microalgae.
Highlights
Recent studies have demonstrated that microalga has been widely regarded as one of the most promising raw materials of biofuels
Flocculation of microalgal cells by pH decrease Flocculation efficiencies for the three species of freshwater microalgal cells were studied in terms of pH variations (Figure 1)
The microalgal cells began to coagulate when the pH decreased from pH 6.7 to about 5.0 Coagulating of algal cells could still be observed when pH further decreased to 4.5, but the coagulated cells remained suspended in the growth medium
Summary
Recent studies have demonstrated that microalga has been widely regarded as one of the most promising raw materials of biofuels. Many methods of harvesting microalgae, including mechanical, electrical, biological and chemical based, have been studied to overcome this hurdle. Microalga is an economical and potential raw material of biomass energy [3], because it does not require a large area of land for cultivation, exhibits short growth period, possesses a high growth rate and contains more high-lipid materials than food crops [4,5,6]. There are currently several harvesting methods, including mechanical, electrical, biological and chemical based [11]. Microalgal cells are harvested by mechanical external forces,such as centrifugation [12], filtration [13], sedimentation [14], dissolved air flotation [15,16] and usage of attached algae biofilms and ultrafiltration membranes [17,18]. Electrolytes and synthetic polymers are typically utilized [23]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
