Abstract
Neonatal sepsis is a significant problem in developing nations, but current gold-standard diagnostic methods, such as blood culture, is slow and time-consuming. Here, we describe the development of a colorimetric loop-mediated isothermal amplification (LAMP) assay that targets the wabG gene in the lipopolysaccharide region of K. pneumoniae, offering a limit of detection (LOD) of 40 CFU/ml with specificity for K. pneumoniae compared to other non-Klebsiella strains. The sensitivity and specificity of the LAMP assay were found to be 90 % and 100 %, respectively, with a positive predictive value of 100 % and a negative predictive value of 96.47 %. The LAMP assay demonstrated a significantly shorter turnaround time of 1 h. The LAMP assay was found to be simpler, quicker, and more sensitive than traditional detection techniques such as PCR and blood culture.
Published Version
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