Abstract
The performance of the Reflotron system (Boehringer Mannheim) for the determination of urate in whole blood and serum was evaluated. Within-run and day-to-day imprecision of the system were comparable with those for a solution-chemistry enzymatic method (overall CVs in the range 2.2-2.5%). Results for 100 individual specimens with urate concentrations ranging from 16 to 134 mg/L agreed well with the comparison method, both for serum and whole blood. We saw no significant interference from lipemia or hemoglobin. Bilirubin interfered at concentrations greater than 100 mg/L. Hematocrit variation between 25% and 55% did not affect results for whole blood; variation of the applied sample volume from 28 microL to 35 microL (stated sample volume requirement: 30 microL) did not significantly influence the measured value. We consider results produced by the system to be of the same analytical quality as those obtained by the more conventional solution-chemistry methods that are currently available.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
