Abstract

Cosmetic products are those formulations which are applied on different parts of body including teeth, nails, face and hair. These are used to clean body and improve appearance. On the basis of area on which cosmetics are applied these can be categorized in different categories like cosmetics for oral care, for skin, for hair and perfumes etc. Microorganisms are known for so many years due to their ability to grow and multiply in cosmetic care products. Cosmetic products are not considered to be sterile but there must be a minimum level of microbial contamination. This study was conducted to assess the cosmetic care products for their potential microbial contaminants (both fungal and bacterial). Cosmetic products were collected in two categories; cosmetic creams and moisturizing lotions. Pour plate technique was used to determine total viable count. Sample dilution was made in Phosphate buffer saline containing 0.5% of an emulsifying agent that was polysorbate 80. Different general purpose, selective and differential media were used for culturing. Identification was based upon morphology of colonies, Gram reaction and standard biochemical tests. Antibiotic sensitivity was checked by using different antibiotic and antifungal discs on Muller-Hinton Agar and Sabouraud Dextrose Agar. In this study the contaminants isolated from creams and lotions were; Pseudomonas aeruginosa, E. coli, Staphylococcus aureus and Aspergillus niger. Among these isolates P. aeruginosa, S. aureus and E. coli were sensitive to Ciprofloxacin, Amikacin and Tobramycin while A. niger was sensitive to Streptomycin, Ketoconazole and Clotrimazole.

Highlights

  • In our society beauty standards are becoming very high due to the placement of photo shopped advertisements of models

  • The contaminants isolated from creams and lotions were P. aeruginosa, S. aureus, E. coli and A. niger

  • P. aeruginosa, S. aureus and E. coli were sensitive to ciprofloxacin amikacin and tobramycin while A. niger was found sensitive to all three drugs used in this study

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Summary

INTRODUCTION

In our society beauty standards are becoming very high due to the placement of photo shopped advertisements of models. Present mechanical barriers and different mechanisms of defense protect human skin and mucosal membrane from microbial attack. This defense mechanism may get disturbed due to the application of cosmetic products. The easiest way to evaluate risk factors related to the use of cosmetic products is to assess their exposure with the skin Use of these beauty products is different according to age groups and geographical area. Formaldehydes are added in a very minute amount which does not cause any harmful effects unless used repeatedly By using such products for long time, different sensitivity reactions may occur. Presence of microorganisms like; E. coli, Staphylococcus aureus, Clostridium spp., Salmonella spp., and Pseudomonas aeruginosa is strictly avoided [19]

Sample Collection
Sample Processing
Pour plate technique
Viable microbial count
Microbial identification
Pure Culture
Antibiotic Susceptibility
RESULTS AND DISCUSSION
Antibiotic and Antifungal Sensitivity
CONCLUSION
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