Evaluation of Central Nervous System Acting Effects of Citrus Peel Essential Oils Extracted Using Enzyme Technology on Rodent Models

Abstract

Abstract-There is growing evidence of measurable effects of essential oils in animal brains and so more clinical research is required to validate their influence on the human central nervous system. This will enable us to discover essential oil-based drugs for treatment of mental illnesses such as depression, anxiety etc. Several methods have been developed to obtain oil from oil-rich plant materials using aqueous enzymatic methods. By using enzymes to mediate the extraction, it is possible to maintain mild conditions and effect superior extraction. The enzymes such as cellulase, hemicellulase, pectinase and protease are the most favourable enzymes for degrading the cell wall in oilseeds to loosen oil sacs embedded in the seed structures. Reduced equipment costs and energy consumption are also potentially possible, since oil and protein may be recovered simultaneously Therefore, the present study is proposed on quality and quantity enhancement of essential oils from citrus peel waste through enzymatic intervention and evaluates their effect on the central nervous system in Rodent Models. As an attempt to obtain the essential oil through hydro distillation HD and hydro distillation enzyme assisted HDEA was carried out. Several experiments have been conducted to determine the optimal process parameters for both methods, i.e., substrate to solvent ratio, extraction temperature, extraction time, enzyme loading, and incubation time etc. to Obtain essential oil from citrus peel collected from the local market of Dehradun. Total yield and physical characteristics like specific Gravity, viscosity, refractive index, acid, Saponification, iodine no etc. were compared. Both samples of oil CA-1 and CA-2 were further screened for their effect on Central Nervous System on the rodent model. Result indicated HDEAnot only improved yield but also has sustainably stimulant effect on the central nervous system as compared to HD. Keywords: Essential oil, Enzymes, Central nervons system.