Evaluation of Biocompatibility Properties of Dental Materials: xCELLigence® System

Abstract

The toxic and biological impacts of dental materials play a pivotal role in their clinical application within dentistry. The assessment of these materials typically commences with in vitro tests upon initial development, progressing to in vivo animal experiments and clinical trials. In vitro cell culture tests afford the examination of tissue responses at the cellular level, allowing the observation of physiological activities. Moreover, these tests offer a cost-effective and time-efficient alternative to animal experiments, rendering them easily applicable and replicable. Recently, real-time cell analysis systems, such as the xCELLigence® system, have emerged as a promising substitute for traditional testing methods, potentially surpassing them in the biocompatibility evaluation of dental materials. The xCELLigence® system facilitates the concurrent observation and analysis of cells within their authentic environment, obviating the need for cell staining or marking. This review seeks to underscore the advantageous features of the xCELLigence® system, which serves to mitigate the drawbacks associated with conventional in vitro biocompatibility evaluation methods.