Abstract
Flatfish caught at four different sites in the Hvaler Archipelago were analysed for content of organochlorine contaminants (polychlorinated biphenyls PCB, hexachlorobenzene, HCB, 1,1-dichloro-2,2-bis( p-chlorophenyl)ethylene, DDE, polychlorinated dibenzofurans, PCDF, and polychlorinated dibenzodioxins, PCDD) and polyaromatic hydrocarbons, PAH, in fillet and liver, and for cytochrome P-450-dependent (ethoxyresorufin O-deethylase, EROD, and ethoxycoumarin O-deethylase, ECOD) and conjugating (UDP-glucuronyltransferase) enzyme activities in liver S9-fractions. The liver samples were also analysed immunochemically using Western blotting and an enzyme-linked immunosorbent assay (ELISA) with antibodies against the β-naphthoflavone (BNF)-inducible cytochrome P-450 forms from cod, rainbow trout and perch. Sites close to contaminant effluents showed elevated levels of the organochlorines HCB, DDE and PCB, but only background levels of PCDF/PCDD and PAH in sampled fish. Some of these sites showed highly induced EROD activities in the fish. Of the flatfish caught, only dab ( Limanda limanda) showed a good correlation between EROD activity and immunochemical cross-reactivity.
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