Abstract

Mycoplasma gallisepticum and M. synoviae membranes were obtained by lysing mycoplasma cells with carbonate bicarbonate buffer at pH 10. Membrane preparations were compared with whole-cell preparations as to efficacy in the plate and tube agglutination and hemagglutination-inhibition tests. Membrane antigens were somewhat more sensitive in the plate and tube agglutination tests, whereas the hemagglutination-inhibition test showed no major difference between whole and membrane antigens. The membrane preparations were equal to whole-cell antigens in specificity.

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