Evaluation of attachment of osteoblast-like cells based on morphology at the fixture surface of dental implants

Abstract

Background and Aim: Bacteria can attach to the implant surfaces, and the rough pattern can make it difficult to clean the microorganisms and result in an inflammatory process and loss of the implant. Accordingly, the present study aimed to investigate the attachment of osteoblast-like cells based on morphology at the fixture surface of dental implants. Materials & Methods: MTT test (3- [4,5-Dimethylthiazol-2-yl] -2,5-Diphenyl Tetrazoliumbromide) was used for quantitative evaluation of the effect of 980nm Diode laser irradiation and Er: YAG on the viability rate of saos-2 cells on implants. In this method, the cell viability rate is evaluated by quantifying a metabolic product and confirms the mitochondrial activity of cells, which is directly associated with cell growth and, subsequently, its viability. Results: The results of this study show that based on the MTT test, the percentage of viability of cells for implants under Diode laser irradiation was higher than the control group, but this value was not significant. This value was lower than the Er: YAG laser, which was insignificant. Conclusion: According to SEM observations, the density of cell aggregates and surface spreading of the implants were similar in the three groups.