EVALUATION OF ANTI-ULCER ACTIVITY OF AQUEOUS EXTRACT OF BLACK CUMIN SEEDS (NIGELLA SATIVA L) ON EXPERIMENTAL ALBINO RATS.

Abstract

: Seeds of Nigella sativa L. (Rananculacene) or Black cumin seed is widely used in the Indian subcontinent in treatment of gastric ulcer as a traditional medicine. Aim: To investigate gastro-protective activity of aqueous extract of black cumin seeds in aspirin induced ulcer models in albino rats taking ranitidine as positive control. Settings and Design: Albino rats of Wistar strain, (150-200 g) was divided randomly into four groups (n=6) and received the following medications orally for five days. Group A -1% gum acacia 5 ml/kg, Group B and Group C– aqueous extract of Black Cumin 250 mg/kg and 500 mg / kg body wt respectively and Group D - Ranitidine 20 mg / kg body wt; followed by aspirin 200mg/kg body wt administered orally on 6th day to all groups after 24 hours of fasting. Methods and Materials: Animals were sacrificed after 4 hours of pyloric ligation. The stomachs were removed. The gastric contents were evaluated by biochemical parameters and gastric ulceration was studied by comparing the volume of gastric juice, free acidity, total acidity, ulcer index and by histopathological study. Statistical analysis used: Statistical analysis was done using one way ANOVA and Tukey Kramer Multiple Comparison Test. Results: Aqueous extract of N. Sativa in doses 250mg/kg and 500mg/kg reduced volume of gastric juice, free and total acidity and ulcer index in a highly significant manner when compared to control (p<0.001). N.sativa (500mg/kg) dose proved to be comparable (p>0.05) to positive control (ranitidine 20 mg/kg), while N. sativa 250mg/kg proved to be less effective than its higher dose and ranitidine (p<0.01). The results were expressed as mean ± SEM Conclusion: Aqueous extract of NS (500mg/kg) has potent gastroprotective properties comparable to Ranitidine 20mg/kg. Key Words; Black cumin, Nigella sativa (Ns), antiulcer.