Abstract
Objectives: This study was aimed at scientifically evaluating the in vitro antipsoriatic activity of Siddha drug Panchamuga Chendhuram (PMC) in human keratinocyte (HacaT) cell lines.
 Methods: The Siddha drug PMC tested for antipsoriatic activity on HacaT cell lines was morphologically examined by phase contrast microscopy, and the cell viability was determined by 3- (4, 5 dimethyl thiazole-2 yl) -2.5-diphenyl tetrazolium bromide assay. About 100 μl of different concentrations (2, 6, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 μg/ml) of the test samples were prepared in the cell culture medium and incubated for 24 h and 48 h to determine the viable cells.
 Results: The results revealed that Siddha drug PMC showed hopeful antiproliferative activity. In vitro studies showed that after 24 h and 48 h incubation, the inhibitory concentration 50 (IC50) values of PMC (IC50 20 μg/ml) were 72.08±27.56 μg/ml and 43.91±17.71 μg/ml, respectively, as compared with Asiaticoside as a positive control with an IC50 value of 20.13 μg/ml.
 Conclusion: Thus, this study provides scientific evidence about the efficacy of the Siddha drug PMC against the HacaT cell lines confirming its traditional use in psoriasis treatment and also emphasizes the need for antipsoriatic evaluation in animal models.
Highlights
Psoriasis is a serious global problem for many decades
Panchamuga Chendhuram (PMC) is a Siddha Herbomineral formulation used in this study indicated for psoriasis in the classical Siddha textbook Pulippani Vaidhyam – 500 [10]
The proportion of dead cells and type of cell death after treatment was better illustrated from the morphological observations (Fig. 1)
Summary
Psoriasis is a serious global problem for many decades. Its prevalence rate ranges from 0.44% to 2.8% of the Indian population [1]. The name psoriasis was given by the Viennese dermatologist Von Hebra. The name is derived from the Greek word psora which means to itch [2]. Psoriasis is defined on a clinical basis as chronic, relapsing, remitting papulosquamous eruption with typical localization on the extensor surfaces such as elbows and knees involving scalp, genitalia or nails, and other sites [3]. The ratio of male to female was 1.1:1. 12.5% had a family history of psoriasis [4]. Onset disease that affects patients at age
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