Abstract
The antioxidant potential of Heracleum Lasiopetalum extract (GE) as a natural antioxidant in multilayer nanoemulsion was measured. GE obtained using an ultrasonic bath. Extraction efficiency and the total phenolic compounds of the extract were 5.12% and 84.17 μg GA/mg extract, respectively. Antioxidant activity of 200, 400, 600, 800, and 1000 ppm of GE measured by DPPH free radical scavenging methods and, iron reduction assay, then compared with the 100 ppm of TBHQ synthetic antioxidant. The results showed that the antioxidant activity increased with increasing the concentration of the extract in both methods. Three optimized water-in-oil in water emulsion (W/O/W) produced according to the encapsulation efficiency, particle size and zeta potential of nanocapsules as responses of surface methodology as follows: (Lepidium sativum seed gum (emulsifier: 36.30%, oil: 50.46% and extract: 9.52%), whey protein concentrate (emulsifier: 30.85%, oil: 57.07% and extract: 7.12%) and MIX (emulsifier: 39.87%, oil: 54.98% and extract: 9.05%)). Antioxidant activity of 800 ppm of the free and encapsulated extract in different coatings materials compared to SFO containing TBHQ (TBHQ) and SFO without antioxidants (control) stored at 60 °C for 24 days. The oxidation indexes of oil samples include peroxide value, thiobarbituric acid value, and p-anisidine value measured at four-days intervals. The GE encapsulated in whey protein concentrate coating showed less oil oxidation than control and TBHQ samples. These results exhibited that whey protein concentrate is the high-performance coating for encapsulating GE and it can be used as a natural antioxidant and alternative for synthetic antioxidants.
Published Version
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