Evaluation of Antioxidant and Cytotoxic Effects of Liposomes Containing Pineapple Fruit Extract on Melanoma Skin Cancer (A375 Cell Line)

Abstract

Introduction: The use of The use of nanoparticles containing antioxidant and cytotoxic plant compounds can have a special place in the treatment of melanoma. The aim of this study was to evaluate the antioxidant and cytotoxic effects of pineapple fruit extract on skin cancer. Methods: In the present experimental study, liposomal vesicles were prepared using cholesterol, soy phosphatidylcholine, and polyethylene glycol, and pineapple fruit extract was loaded in liposomes. Physicochemical characteristics were evaluated using zeta sizer, FTIR and AFM. Finally, the toxicity of different concentrations of extract and liposome-containing extract was evaluated in A-375 melanoma cell line using MTT assay. DPPH test was used to evaluate the antioxidant properties of the extract and liposomes containing extract. Statistical analysis was performed using Excel and SPSS (Ver 22) software and Duncan and Student's T-tests were used for statistical conclusion. Results: According to this study showed the encapsulation efficiency of pineapple extract containing liposome, liposome size and its surface charge were 40%, 89.9 nm and -8.8 mV, respectively. FTIR analysis and AFM micrographs also confirm that there is no interaction of the extract with its nanosystem, the spherical morphology of the liposomes and its appropriate distribution and dispersion. The toxicity level of pineapple extract is higher when had been encapsulated rather than the non-encapsulated extract on the A-375 cell line. Conclusion: Pineapple fruit extract has cytotoxic effects on A-375 cell line and the present liposomal nanocarrier can be a suitable carrier for the delivery of the extract and inhibit the growth and proliferation of these cells.