Evaluation of Antimicrobial Effects of Euphorbia cyparissias Extracts on Intramacrophages Salmonella typhi

Abstract

Typhoid fever is a disease caused by Salmonella typhi (S. typhi) and one of the most important enteric infectious diseases in the world. Euphorbia cyparissias extracts showed antidiarrhoeic activity and a flavonoid, quercitrin, was isolated from this plant.This study evaluated on antimicrobial effects of Euphorbia cyparissias extracts against intramarophges S. typhi. Euphorbia cyparissias extracts were prepared in ethanol, aceton and distilled water. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) for S. typhi were evaluated by broth macrodilution and agar well diffusion methods. Effect of extracts on intramacrophage surviving of S. typhi studied on cell culture of mouse peritoneal macrophages. MIC values respectively 2.96 to 24.5 mg/ml and MBC respectively 5.93 to 49 mg/ml (in this study acetone, methanol, water). In cell culture results indicated that all extracts were not inhibitory effect on macrophages. The results of intramacrophages study showed a significant decrease in the number of colony forming on the test plates comparing with control group. (p value≤0.05). Overall this study indicated that extracts of Eucalyptus cyparissias have antimicrobial effect against intramacrophage S. typhi and acetonic extract is the most effective antimicrobial activity on intramacrophage S. typhi, therefore these extracts can be useful in treatment of typhoid fever.