Evaluation of Antimicrobial Activity of Two Endodontic Sealers: Zinc Oxide with Thyme Oil and Zinc Oxide Eugenol against Root Canal Microorganisms- An in vitro Study.

Manoj Chandak,Silpi Basak,Nilima Thosar

doi:10.5005/jp-journals-10005-1489

Abstract

AimThe present study was aimed to find out and compare the antimicrobial effect of the paste containing zinc oxide cement mixed with thyme oil (ZO + Th oil) with that of the paste containing zinc oxide and eugenol (ZO + E) against Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, and Pseudomonas aeruginosa, common root canal pathogens of deciduous teeth.Materials and methodsAn in vitro antimicrobial effect was carried out by the agar diffusion method. The ZO + Th oil paste was filled in the punched holes of Mueller Hinton agar at two equidistant points. The same was followed for ZO + E paste. For 24 hours, all the plates were incubated at a temperature of 37°C. The inhibition zones in millimeters around the wells were calculated. There were 6 times repetitions of the test for each microorganism. Data were tabulated and analyzed statistically using one-way analysis of variance (ANOVA) and Tukey’s post hoc comparison test. Level of significance for the tests was 5%.ResultsZones of bacterial inhibition were highest for ZO + Th oil paste against the pathogenic microorganisms S. aureus, followed by E. coli, E. faecalis, P. aeruginosa while for ZO + E paste, the decreasing order against pathogenic microorganisms was E. coli, S. aureus, E. faecalis, and P. aeruginosa. Statistically significant difference was found in both the pastes, ZO + Th oil paste and ZO + E paste.ConclusionZO + Th oil paste showed higher levels of antimicrobial effect against the root canal pathogens.How to cite this article: Thosar NR, Chandak M, Bhat M, Basak S. Evaluation of Antimicrobial Activity of Two Endodontic Sealers: Zinc Oxide with Thyme Oil and Zinc Oxide Eugenol against Root Canal Microorganisms—An in vitro Study. Int J Clin Pediatr Dent 2018;11(2):79-82.

Highlights

Zones of bacterial inhibition were highest for ZO +
Microbial infections in root canals of primary teeth are polymicrobial in nature.[1]
It is important to use an obturating material which has antibacterial properties to act on such microorganisms of inaccessible areas of root canals of primary teeth

Summary

Introduction

Microbial infections in root canals of primary teeth are polymicrobial in nature.[1] Pulpectomy of primary teeth includes biomechanical preparation and use of intracanal dressing with antibacterial properties. The success of endodontic treatment depends on the removal of infected bacteria.[2] There are various methods: proper debridement with instrumentation, antibacterial irrigations, and antibacterial filling materials.[3]. Due to the presence of numerous accessory canals in primary teeth, intracanal dressings may fail to remove these microorganisms from inaccessible areas. It is important to use an obturating material which has antibacterial properties to act on such microorganisms of inaccessible areas of root canals of primary teeth. Thyme oil shows its potential to be used in dentistry.[4]

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