Pages 1-6 Evaluation of Antibacterial Activity of Root Canal Sealers Used in Primary Teeth Against E. faecalis

Abstract

Aim: The aim of this study was to assess the antibacterial activity of seven different root canal sealers used in primary teeth against Enterococcus faecalis. Material and Method: The study was conducted using the agar diffusion inhibitory test. The sealers used were Sure-paste, Metapex, Tg Pex, Calcicure, Endoflas FS, Sealapex, Ca(OH)2 mixed with %2 Chlorhexidine Gluconate solution and one control group material (Klorhex Oral Gel). The injectable form sealers and the freshly-mixed sealers were placed into the prepared wells on the agar plates which were inoculated with the Enterococcus faecalis. After incubation periods of 24 hours, 48 hours, and 72 hours, the zones of the growth inhibition were observed and measured. The average of 8 measurements for each material was taken. The data were compared using one-way analysis of variance (ANOVA) (p<0.05). Results: Even though Sure-paste, Calcicure and Ca(OH)2 powder mixed with Klorhex irrigation solution have shown antibacterial activity against E.faecalis, Metapex, Tgpex, Endoflas FS and Sealapex have not. Calcicure and Ca(OH)2 powder mixed with Klorhex irrigation solution was more effective than Sure-paste (p<0.05). The largest zone of inhibition was found with Klorhex oral gel (control group). But, there was no significant differences between Klorhex oral gel, Calcicure and Ca(OH)2 powder mixed with Klorhex irrigation solution. There were changes in the antibacterial activity of Sealapex from the 24-h period through the 48-h period and 24-h period through the 72-h period. Conclusion: Based on the results of this study it can be concluded that Ca(OH)2/Chlorhexidine gluconate and injectable Ca(OH)2 pastes more effective than Ca(OH)2, iodoform and zinc oxide eugenol mixtures.