Abstract
The main aim of this study is to evaluate the comparison of antimicrobial efficacy of silver nanoparticles synthesized from aqueous plant extracts of Phyllanthus amarus and Tinospora cordifolia. The synthesized silver nanoparticles were characterzed by UV-VIS spectroscopy, Fourier Transform Infra-Red spectroscopy, Transmission Electron Microscopy, Dynamic Light Scattering and zeta potential. The antimicrobial activity of synthesized silver nanoparticles was compared with their respective plant extracts by agar well diffussion method and minimum inhibitory concentration was also calculated. The zone of inhibition varied in range of 12 ± 1 to17 ± 0.58 mm with 100 μg/ml silver nanoparticles concentration, while acetonic, methanolic and aqueous extracts of respective plants does not showed any activity even at 1 mg/ml i.e. 10 times more than that of silver nanoparticles. MIC of silver nanoparticles was found to be in a range of 6.25-25 μg/ml against all tested microbes. The antimicrobial activity of synthesized silver nanoparticles was higher than that of the standard drug i.e. streptomycin (for bacteria) and ketoconazole (for fungus). The synthesized nanoparticles of P. amarus and T. cordifolia have shown good antimicrobial efficacy as compared to plant extracts and may prove to be better antimicrobial agent against wide range of microbes.
Highlights
A large number of plants are being used in medicine for therapeutic and prophylactic purposes
We have synthesized the AgNPs from aqueous extracts of P. amarus and T. cordifolia
Further we evaluated the comparison of antibacterial activity of AgNPs with plant extracts against reference ATCC bacterial and fungal strains
Summary
A large number of plants are being used in medicine for therapeutic and prophylactic purposes. The beneficial medicinal effects of plant products typically result from the combinations of secondary metabolites present in the plants. The therapeutic properties of medicinal plants are attributed owing to the presence of active substances such as alkaloids, flavonoids, glycosides, vitamins, tannins, and coumarins [1]. These affect the body of human beings, interact with the pathogens and interrupt their growth at different stages of development and make the body disease free. Silver has long been recognized as having inhibitory effect on microbes present in medical and industrial process [2]. There is a growing demand for the devising of environmentally agreeable protocols for the synthesis of nanomaterials that would avoid the hazardous by-products associated with current physicochemical processes [4,5]
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