Evaluation of Antibacterial, Antioxidant and Immunomodulatory Potential of Mitragyna parvifolia Leaves-Assisted Fe2O3 Nanoparticles

Abstract

This work includes the use of leaves of Mitragyna parvifolia for green synthesis of Fe2O3 nanoparticles (Fe2O3 NPs), which offers a sustainable eco-friendly approach. Characterization of synthesized Fe2O3 NPs was done by UV-visible, FTIR spectroscopy, XRD and FESEM studies. FTIR exhibited the existence of Fe-O, O–H and COO–Fe stretching owing to the occurrence of the carboxylic group. FESEM analysis revealed that the average size of Fe2O3 NPs was 92 nm, which varies in their shape. The study also includes the determination of antibacterial, in vitro antioxidant and immunomodulatory efficacy of synthesized Fe2O3 NPs. Results suggested that Fe2O3 NPs exhibit potent antibacterial efficacy against B. subtilis and S. aureus, gram-positive as well as E. coli and P. aureginosa, gram-negative bacterial strains used in this study. The study exhibited significant (p < 0.01) in vitro antioxidant and immunomodulatory potential of synthesized Fe2O3 NPs against concanavalin-A activated Wistar albino rat’s splenocytes. The green synthesized Fe2O3 NPs using M. parvifolia leaves may offer a novel approach in the medicinal and immunological field to overcome oxidative stress as well as enhance immune responses for potential health benefits of human beings.