Evaluation of anti-inflammatory and healing activity of a nano-structured lipid carrier containing tucuman butter oil and butter

Aline Rossato,Roger Wagner,Pâmella Schramm Oliveira,Bruna Klein,Diego De Souza,Larissa Da Silva Silveira,Walter Paixão De Souza Filho,Michele Rorato Sagrillo,Matheus Dellaméa Baldissera

doi:10.37779/nt.v21i3.3551

Abstract

The Amazon region has a wide variety of native palm trees that have fruits and oilseeds. Among these palm trees, Para tucuma (Astrocaryum vulgare) stands out, which provides the tucuma fruit from which its oil is extracted, which has anti-inflammatory, antioxidant, healing and antibacterial properties, among others. However, oils, like that of tucuma, are sensitive to thermal degradation, as well as oxidative reactions, which can reduce their pharmacological effectiveness. In this way, nanotechnology can be a tool to preserve the pharmacological properties of essential oils and increase their therapeutic effectiveness. Therefore, this study aims to develop a nanostructured lipid carrier (NLC) containing tucuma butter and oil, to analyze its in vitro properties of anti-inflammatory and healing activity. For that, tests of anti-inflammatory activity and healing activity were performed by the Scratch test in vitro. The results of the tests demonstrated that the majority of the NLC concentrations showed anti and pro-inflammatory and healing activity.

Highlights

Bioactives from fruits and vegetables are receiving great attention due to their low cost and wide margin of safety in addition to therapeutic efficacy (SOUZA FILHO et al, 2012)
The diameter and polydispersity index (PDI) determinations of the nanoparticles were performed through dynamic light scattering; the zeta potential was measured by electrophoresis (Zetasizer® nano-Zs model ZEN 3600, Malvern), the determination of the pH of the nanoparticles was carried out in pH-meter
When evaluating the nanostructured lipid carrier (NLC) formulations under different conditions of temperature and storage, it can be observed that there were no significant changes in the stability of the formulations during the period of 60 days, in any of the conditions of storage and temperature that they were exposed to

Summary

Introduction

Bioactives from fruits and vegetables are receiving great attention due to their low cost and wide margin of safety in addition to therapeutic efficacy (SOUZA FILHO et al, 2012). With scientific progress, involving chemical and pharmacological studies of medicinal plants, new bioactive agents with therapeutic properties were obtained (MACEDO et al, 2017). In this context, tucumã is found, whose fruits are used for food and is considered a rich source of bioactive, has a high content of lipids, a large amount of carotene, a precursor to vitamin A, high levels of fiber and vitamin E. In order to reduce or prevent the loss of pharmacological activity of a substance, there was a need to develop mechanisms to increase the efficiency of formulations. NLC can be used in various formulations, thanks to its physical, aesthetic and sensorial structure, being excellent means of carrying substances, promoting an increase in bioavailability, solubility and protection of the physical-chemical properties of the asset (ATTAMA et al, 2012)

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Results