Abstract
Introduction: Nanotechnology is a rapidly developing interdisciplinary area that has brought enormous changes in dentistry. Copper nanoparticle made from plant extract would be an environmental friendly, convenient and dependable way for providing therapeutic agents that are safe, free of side effects and useful for a wide range of diseases. Mucuna pruriens seed extract was selected for our study, due to its due to its anti- bacterial, anti-diabetic, anti- Parkinson, anti-cholesterol and anti- oxidant properties.
 Aim: To evaluate the anti-inflammatory properties of Mucuna pruriens, the green synthesis, characterization of CuNPs, and screening of their cytotoxic activity.
 Materials and methods: The collection and preparation of Mucuna pruriens extract was done and stored for further use. Synthesis of Cu nanoparticles was done with 30 milli molar of copper (II) sulfate. Centrifugation was done and characterisation of Copper nanoparticles using ultraviolet (UV)-visual spectrophotometer. Cytotoxic effect and anti-inflammatory activity of copper nanoparticles with Mucuna pruriens seed extract were assessed using Brine Shrimp Assay at 5µL, 10µL, 20µL, 40µL and 80 µL and Bovine Serum Albumin (BSA) at 5 µL, 10 µL, 20 µL, 30 µL, 50 µL.
 Results: The anti inflammatory activity of Copper Nanoparticles with Mucuna pruriens, increased with increase in concentrations. Percentage of inhibition was 17% at 10 µL concentration, 24% at 20 µL, 43% at 30 µL and 54% at 40 µL and highest at 50 µL (71%). Cytotoxicity of Copper Nanoparticles with Mucuna pruriens, at 5 µL concentration there was a death of 10% of nauplii, at 10 µL there was a death of 20% of nauplii, at 20 µL and 40 µL there was a death of 30% of nauplii and at 80 µL there was a death of 40% of nauplii. As the concentration increased, the cytotoxicity of the nanoparticles increased.
 Conclusion: Based on the results of the current study, it is concluded that Mucuna pruriens mediated Cu Nps can be used as a potential source of anti inflammatory agent and also as an anti cancer drug for the treatment of tumours and cancers.
Highlights
Nanotechnology is a rapidly developing interdisciplinary area that has brought enormous changes in dentistry
Cytotoxicity of Copper Nanoparticles with Mucuna pruriens, at 5 μL concentration there was a death of 10% of nauplii, at 10 μL there was a death of 20% of nauplii, at 20 μL and 40 μL there was a death of 30% of nauplii and at 80 μL there was a death of 40% of nauplii
Based on the results of the current study, it is concluded that Mucuna pruriens mediated comparison between the Mucuna pruriens (Cu Nps) can be used as a potential source of anti inflammatory agent and as an anti cancer drug for the treatment of tumours and cancers
Summary
Nanotechnology is a rapidly developing interdisciplinary area that has brought enormous changes in dentistry. One of the most challenging nanoscale researches in recent years has been the synthesis of green chemistry and nanotechnology [6] These studies can be useful in the field of nanomedicine for the upcoming generation due to their low cost and extra revenue[7]. Cu nanoparticles is an essential trace element in living organisms, which plays an important role in protein function These nanoparticles (CuNPs) have shown high toxicity against tumor cells such as pulmonary adenocarcinoma (A549) and human leukemia monocytic cell lines (THP-1)[9][10]. The main purpose of cancer chemotherapy is to kill cancer cells directly while avoiding toxicity of healthy cells This is the limitation to the use of several chemotherapeutic agents[16]. Selective toxicity must be put in consideration in the discovery of leads for cancer treatment
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