Evaluation of Anti Inflammatory Activity of Hydroalcoholic Leaves Extracts of Polyherbal Combination of Vitex Negundo and Murraya Koenigii against Carrageenan Induced Paw Edema in Rats

Abstract

Vitex Negundo and Murraya Koenigii are popular Indian herbs which are used for various clinical disorders. The basic literature survey exposed that Vitex Negundo hasantioxidant, antiulcer and anti-nociceptive activity and Murraya Koenigii possessing anthelmintic, analgesic, cooling activity, but not many scientific studies have been carried out to reveal and collaborate the anti-inflammatory activity of Vitex Negundo and Murraya Koenigii leaves. All animals were divided into five groups, six rats in each group. Group I was treated as toxicant control to observe swelling, group II was treated with Aspirin (10 mg/kg, p.o), group III and IV were treated with hydroalcoholic extract of leaves of Vitex Negundo and Murraya Koenigii at two doses (100 mg/kg, p.o.) respectively. The anti-inflammatory activity was studied by using carrageenan induced paw edema in rats. Present study showed that hydroalcoholic leaves extract of Vitex Negundo and Murraya Koenigii significantly reduce paw edema when compared with group I (Toxicant control) rats and the result were dose dependent. The toxicity study also revealed its safeness, thus the plant can be hypothesized it is nontoxic. All data were expressed as mean+SEM and were statistically analysed one way ANOVA. It is concluded that hydroalcoholic leaves extract of Vitex Negundo and Murraya Koenigii can offer protection against carrageenan induced paw edema in rats.